Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
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DOI：
10.1371/journal.pone.0259895
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Han Y
中科院分区：
文献类型：
--
作者：
Han Y
吸烟可增加龋病的发病率，但吸烟与致龋生物膜生长之间的关系研究较少。本研究旨在探讨CS暴露对变形链球菌生物膜生长和毒力的影响。变种人）。简而言之，S。分别暴露于CS 1、3和6次/d，在唾液包被的羟基磷灰石盘上形成变形杆菌生物膜。此外，S.未暴露于CS的变形杆菌生物膜被视为对照组。分析了产酸性、干重、集落形成单位（CFU）、水溶性/不溶性胞外多糖（EPS）和胞内多糖（IPS），并利用共聚焦激光扫描显微镜（CLSM）对74 h龄S.获得了变形菌生物膜。与对照组和其他CS暴露组相比，6次/d CS暴露组74 h龄S的生物膜和EPS积累最低。变形杆菌生物膜CLSM还揭示了最低的细菌计数（活细胞和死细胞）和EPS生物量在6次/天的CS暴露组在74小时的S。变形杆菌生物膜CS暴露抑制S.在体外研究中，抗致龋生物膜形成的增强具有剂量（频率）依赖性，在该频率下，本研究结果具有更大的影响。
The increased incidence of dental caries by cigarette smoking (CS) has been widely reported in epidemiological studies, but the relationship between CS and cariogenic biofilm growth has been rarely studied. This study aims to investigate the effects of CS exposure on the growth and virulence of Streptococcus mutans biofilms (S. mutans). Briefly, S. mutans biofilms were formed on saliva-coated hydroxyapatite disks, which were exposed to CS 1, 3, and 6 times per day, respectively. In addition, S. mutans biofilms without CS exposure were considered as the control group. Acidogenicity, dry weight, colony-forming units (CFUs), water-soluble/insoluble extracellular polysaccharides (EPSs), and intracellular polysaccharides (IPSs) were analyzed and confocal laser scanning microscopy (CLSM) images of 74-h-old S. mutans biofilms were obtained. The lowest accumulation of biofilms and EPSs were detected in the 6 times/day CS exposure group compared with those of the control group and other CS exposure groups in 74-h-old S. mutans biofilms. CLSM also revealed the lowest bacterial count (live and dead cells) and EPSs biovolume in the six times/day CS exposure group in 74-h-old S. mutans biofilms. CS exposure inhibited the growth of S. mutans biofilm in vitro study, the anti-cariogenic biofilm formation was enhanced with a dose (frequency)-dependent at which frequency has more influence in the present findings.
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影响因子：
3.2
作者：
Koo, H.;Xiao, J.;Jeon, J. G.
通讯作者：
Jeon, J. G.
影响因子：
4.2
作者：
Takahashi, N.;Nyvad, B.
通讯作者：
Nyvad, B.
影响因子：
4.4
作者：
Nobre, Miguel de Araujo;Malo, Paulo
通讯作者：
Malo, Paulo
DOI：
10.3390/ijerph16101669
发表时间：
2019-05-02
影响因子：
--
作者：
Cuadra, Giancarlo A.;Smith, Maxwell T.;Palazzolo, Dominic L.
通讯作者：
Palazzolo, Dominic L.
影响因子：
3.2
作者：
Reibel, J
通讯作者：
Reibel, J