Starvation/stationary-phase survival of Rhodococcus erythropolis SQ1: a physiological and genetic analysis
Starvation/stationary-phase survival of Rhodococcus erythropolis SQ1: a physiological and genetic analysis
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红平红球菌 SQ1 的饥饿/稳定期存活：生理和遗传分析
作者：
Nicolas V. J. Fanget;S. Foley
红串红球菌SQ 1的适应能源和碳饥饿的生存能力饥饿和营养诱导的稳定期的贡献交叉保护其他类型的环境压力方面进行了研究。结果表明，R.红串菌SQ 1在LB和蒸馏水中存活至少43天，在含有高（1%）或低（0.1%）葡萄糖的化学成分确定的培养基（CDM）中存活65天。此外，早期静止相R.与指数期细胞相比，在CDM 0.1%中生长的红串菌SQ 1表现出增强的耐热性和氧化应激性。本研究的第二个目的是确定参与饥饿/静止期生存的遗传因素。R.随机转座子插入突变产生的红串菌SQ 1进行筛选;当在CDM 1%中生长时，四个突变体失去可培养性。当这些突变体在CDM 0.1%中生长时，未观察到可培养性下降。从三个突变体中可以回收DNA侧翼转座子插入。转座子插入被发现在uvrB（UvrB，DNA切除修复机制的一部分），之间的一个假定的guaB基因和另一个guaB样基因，和之间的一个基因编码一个假定的磷酸甘油酸酯酶和假定的硫氧还蛋白/细胞色素c生物合成基因。这是第一次研究的饥饿/静止期的生存反应的红球菌，一个有机体的巨大意义，在环境生物修复和一些工业过程。
The adaptation of Rhodocccus erythropolis SQ1 to energy and carbon starvation was investigated in terms of both the capacity to survive starvation and the contribution of a nutrient-induced stationary phase to cross-protection to other types of environmental stress. It was found that R. erythropolis SQ1 survives for at least 43 days in LB and distilled water, and 65 days in chemically defined medium (CDM) containing high (1%) or low (0.1%) glucose. Furthermore, early stationary-phase R. erythropolis SQ1 grown in CDM 0.1% exhibited enhanced resistance to heat and oxidative stress compared with exponential-phase cells. A second objective of this study was to identify genetic elements involved in starvation/stationary-phase survival. A mutant bank of R. erythropolis SQ1 generated by random transposon insertion mutagenesis was screened; four mutants lost culturability when grown in CDM 1%. No drop in culturability was observed when these mutants were grown in CDM 0.1%. The DNA flanking transposon insertion could be recovered from three mutants. Transposon insertions were found in uvrB (UvrB, part of the DNA excision repair mechanism), between a putative guaB gene and another guaB-like gene, and between a gene encoding a putative phosphoglycerate mutase and putative thioredoxin/cytochrome c biogenesis genes. This represents a first study of the starvation/stationary-phase survival response of Rhodococcus, an organism of immense significance in environmental bioremediation and a number of industrial processes.
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饥饿后恢复生长缺陷的大肠杆菌突变体的分离和表征。
影响因子：
10.5
作者：
Siegele,DA;Kolter,R
通讯作者：
Kolter,R
大肠杆菌 uvrC 基因和蛋白质的序列。
影响因子：
14.9
作者：
Sancar,GB;Sancar,A;Rupp,WD
通讯作者：
Rupp,WD
DOI：
10.1073/pnas.95.18.10716
发表时间：
1998-09-01
影响因子：
11.1
作者：
Goryshin, IY;Miller, JA;Reznikoff, WS
通讯作者：
Reznikoff, WS
影响因子：
15.9
作者：
Mosser, DM;Hondalus, MK
通讯作者：
Hondalus, MK
一种可靠的扩增技术，用于表征插入序列侧翼的基因组 DNA 序列。
影响因子：
2.1
作者：
Prod'hom,G;Lagier,B;Pelicic,V;Hance,AJ;Gicquel,B;Guilhot,C
通讯作者：
Guilhot,C