Sequencing and characterization of Pseudomonas aeruginosa phage JG004.
Sequencing and characterization of Pseudomonas aeruginosa phage JG004.
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DOI：
10.1186/1471-2180-11-102
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发表时间：
2011-05-14
期刊：
影响因子：
4.2
通讯作者：
Schobert M
中科院分区：
文献类型：
--
作者：
Garbe J;Bunk B;Rohde M;Schobert M
噬菌体可能是抗生素的重要替代品，特别是用于治疗多重耐药细菌，例如铜绿假单胞菌。为了有效地使用噬菌体作为抗微生物剂，重要的是要了解噬菌体生物学以及噬菌体感染所必需的细菌宿主的基因。我们分离并鉴定了一种裂解性铜绿假单胞菌噬菌体，命名为JG 004，并对其基因组进行了测序。噬菌体JG 004是肌尾病毒科噬菌体家族的脂多糖特异性宽宿主范围噬菌体。噬菌体JG 004的基因组编码12个tRNA，并且与PAK-P1噬菌体基因组高度相关。为了研究噬菌体生物学和噬菌体-宿主相互作用，我们使用铜绿假单胞菌宿主的转座子诱变并鉴定了噬菌体感染所必需的铜绿假单胞菌基因。对各铜绿假单胞菌突变体的分析揭示了几个特征，如宿主受体和噬菌体JG 004可能的亚精胺依赖性。噬菌体JG 004的全基因组测序结合对感染必需的铜绿假单胞菌宿主基因的鉴定，允许深入了解JG 004生物学，揭示宿主细菌的可能抗性机制，例如LPS和亚精胺生物合成中的突变，并且还可以用于表征铜绿假单胞菌中的未知基因产物。
Phages could be an important alternative to antibiotics, especially for treatment of multiresistant bacteria as e.g. Pseudomonas aeruginosa. For an effective use of bacteriophages as antimicrobial agents, it is important to understand phage biology but also genes of the bacterial host essential for phage infection. We isolated and characterized a lytic Pseudomonas aeruginosa phage, named JG004, and sequenced its genome. Phage JG004 is a lipopolysaccharide specific broad-host-range phage of the Myoviridae phage family. The genome of phage JG004 encodes twelve tRNAs and is highly related to the PAK-P1 phage genome. To investigate phage biology and phage-host interactions, we used transposon mutagenesis of the P. aeruginosa host and identified P. aeruginosa genes, which are essential for phage infection. Analysis of the respective P. aeruginosa mutants revealed several characteristics, such as host receptor and possible spermidine-dependance of phage JG004. Whole genome sequencing of phage JG004 in combination with identification of P. aeruginosa host genes essential for infection, allowed insights into JG004 biology, revealed possible resistance mechanisms of the host bacterium such as mutations in LPS and spermidine biosynthesis and can also be used to characterize unknown gene products in P. aeruginosa.
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影响因子：
3.7
作者：
Kulakov, Leonid A.;Ksenzenko, Vladimir N.;Lavigne, Rob
通讯作者：
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影响因子：
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通讯作者：
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