Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains.
Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains.
复制标题
酿酒酵母麦芽糖代谢全局阻遏蛋白 Tup1 的功能分析：功能域的不同作用。
DOI：
10.1186/s12934-017-0806-6
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发表时间：
2017-11-09
影响因子：
6.4
通讯作者：
Xiao DG
中科院分区：
文献类型：
--
作者：
Lin X;Yu AQ;Zhang CY;Pi L;Bai XW;Xiao DG
Tup 1是酿酒酵母中一个由葡萄糖阻遏、交配型和其他机制协调控制的多个基因家族的通用转录抑制因子。Tup 1的几个功能结构域已被确定，每个结构域对转录抑制具有不同的作用。在这项研究中，我们的目的是调查Tup 1及其结构域在工业面包酵母麦芽糖代谢中的作用。为此，在具有不同遗传背景的工业面包酵母中进行TUP 1基因编码区的一系列读框内截短，并研究麦芽糖代谢、发酵能力、MAL基因表达水平和生长特性。结果表明，TUP 1基因是必需的麦芽糖代谢在工业面包酵母。重要的是，Tup 1的不同结构域在工业面包酵母细胞的葡萄糖抑制和麦芽糖代谢中发挥不同的作用。Ssn 6相互作用、N端阻遏结构域和C端阻遏结构域可能参与了Tup 1对面包酵母麦芽糖代谢MAL转录的调控。缺失第一个重复序列的WD区可能直接影响麦芽糖代谢的调控，而不是通过葡萄糖阻遏间接影响。这些研究结果为优化工业面包酵母菌株以加速麦芽糖代谢奠定了基础，并促进了未来对其他糖代谢中葡萄糖阻遏作用的研究。本文的在线版本（10.1186/s12934-017-0806-6）包含补充材料，可供授权用户使用。
Tup1 is a general transcriptional repressor of diverse gene families coordinately controlled by glucose repression, mating type, and other mechanisms in Saccharomyces cerevisiae. Several functional domains of Tup1 have been identified, each of which has differing effects on transcriptional repression. In this study, we aim to investigate the role of Tup1 and its domains in maltose metabolism of industrial baker’s yeast. To this end, a battery of in-frame truncations in the TUP1 gene coding region were performed in the industrial baker’s yeasts with different genetic background, and the maltose metabolism, leavening ability, MAL gene expression levels, and growth characteristics were investigated. The results suggest that the TUP1 gene is essential to maltose metabolism in industrial baker’s yeast. Importantly, different domains of Tup1 play different roles in glucose repression and maltose metabolism of industrial baker’s yeast cells. The Ssn6 interaction, N-terminal repression and C-terminal repression domains might play roles in the regulation of MAL transcription by Tup1 for maltose metabolism of baker’s yeast. The WD region lacking the first repeat could influence the regulation of maltose metabolism directly, rather than indirectly through glucose repression. These findings lay a foundation for the optimization of industrial baker’s yeast strains for accelerated maltose metabolism and facilitate future research on glucose repression in other sugar metabolism. The online version of this article (10.1186/s12934-017-0806-6) contains supplementary material, which is available to authorized users.
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MIG1、TUP1 和 SSN6 缺失对瘦面团中麦芽糖代谢和面包酵母发酵能力的影响
影响因子：
6.4
作者：
Lin X;Zhang CY;Bai XW;Song HY;Xiao DG
通讯作者：
Xiao DG
影响因子：
2.5
作者：
HU, Z;NEHLIN, JO;MICHELS, CA
通讯作者：
MICHELS, CA
影响因子：
4.8
作者：
Jabet, C;Sprague, ER;Wolberger, C
通讯作者：
Wolberger, C
DOI：
10.1007/s10295-010-0832-4
发表时间：
2011-01-01
影响因子：
3.4
作者：
Lamas-Maceiras, Monica;Angeles Freire-Picos, Maria;Rodriguez Torres, Ana Maria
通讯作者：
Rodriguez Torres, Ana Maria
影响因子：
3.3
作者：
Green, SR;Johnson, AD
通讯作者：
Johnson, AD