A cis-regulatory change underlying the motor neuron-specific loss of Ebf expression in immotile tunicate larvae.
A cis-regulatory change underlying the motor neuron-specific loss of Ebf expression in immotile tunicate larvae.
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运动神经元特异性EBF表达缺失背后的顺式调节变化。
DOI：
10.1111/ede.12364
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发表时间：
2021-03
影响因子：
2.9
通讯作者：
Stolfi A
中科院分区：
文献类型：
--
作者：
Lowe EK;Racioppi C;Peyriéras N;Ristoratore F;Christiaen L;Swalla BJ;Stolfi A
许多被囊科的物种已经独立地失去了它们的游动幼虫的形态，取而代之的是发育成无尾的、静止的幼虫。这些幼虫不发育游泳所必需的结构，如脊索、耳石和尾部肌肉。然而，人们对这些非游泳性幼虫的神经发育知之甚少。在这里，我们研究了一种非游泳性物种--潜伏牡蛎的运动神经节(MG)的模式。我们发现，与有游动幼虫的物种相比，该物种中MG神经元调节因子的空间模式是保守的，这表明尽管失去了游泳，但调节其表达的基因网络是完整的。然而，关键运动神经元调节基因EBF(Collier/OLF/EBF)在隐蔽分枝杆菌发育过程中的表达与游动幼虫的分枝杆菌相比明显减少。通过测量潜伏支原体与游动种眼镜蛇杂交胚胎中EBF的等位基因特异性表达，证实了这一点。异源报告构建分析在模式被囊藻物种Ciona robusta中发现了一种特定的顺式调控序列变化，该变化降低了EBF在MG中的表达，但在其他细胞中没有。综上所述，这些数据提示隐匿性支原体幼虫中仍有MG神经元，但其分化可能由于EBF表达水平的降低而受损。
Many species in the tunicate family Molgulidae have independently lost their swimming larval form and instead develop as tailless, immotile larvae. These larvae do not develop structures that are essential for swimming such as the notochord, otolith, and tail muscles. However, little is known about neural development in these non-swimming larvae. Here, we studied the patterning of the Motor Ganglion (MG) of Molgula occulta, a non-swimming species. We found that spatial patterns of MG neuron regulators in this species are conserved, compared to species with swimming larvae, suggesting that the gene networks regulating their expression are intact despite the loss of swimming. However, expression of the key motor neuron regulatory gene Ebf (Collier/Olf/EBF) was reduced in the developing MG of M. occulta when compared to molgulid species with swimming larvae. This was corroborated by measuring allele-specific expression of Ebf in hybrid embryos from crosses of M. occulta with the swimming species M. oculata. Heterologous reporter construct assays in the model tunicate species Ciona robusta revealed a specific cis-regulatory sequence change that reduces expression of Ebf in the MG, but not in other cells. Taken together, these data suggest that MG neurons are still specified in M. occulta larvae, but their differentiation might be impaired due to reduction of Ebf expression levels.
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影响因子：
2.4
作者：
Ferrier, DEK;Brooke, NM;Holland, PWH
通讯作者：
Holland, PWH
DOI：
10.1098/rstb.1931.0006
发表时间：
1931-12-01
影响因子：
--
作者：
Berrill, NJ
通讯作者：
Berrill, NJ
影响因子：
2.7
作者：
D'Aniello, Enrico;Pezzotti, Maria Rosa;Branno, Margherita
通讯作者：
Branno, Margherita
影响因子：
4.6
作者：
Imai, Kaoru S.;Stolfi, Alberto;Satou, Yutaka
通讯作者：
Satou, Yutaka
影响因子：
4.6
作者：
Imai, KS;Hino, K;Satou, Y
通讯作者：
Satou, Y