Developing a New qFIBS Model Assessing Histological Features in Pediatric Patients With Non-alcoholic Steatohepatitis.
Developing a New qFIBS Model Assessing Histological Features in Pediatric Patients With Non-alcoholic Steatohepatitis.
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开发一种新的 qFIBS 模型来评估非酒精性脂肪性肝炎儿科患者的组织学特征
DOI：
10.3389/fmed.2022.925357
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发表时间：
2022
影响因子：
3.9
通讯作者：
Zhao, Jing-Min
中科院分区：
文献类型：
--
作者：
Liu, Feng;Wei, Lai;Leow, Wei Qiang;Liu, Shu-Hong;Ren, Ya-Yun;Wang, Xiao-Xiao;Li, Xiao-He;Rao, Hui-Ying;Huang, Rui;Wu, Nan;Wee, Aileen;Zhao, Jing-Min
背景：儿科非酒精性脂肪性肝病(NAFLD)向非酒精性脂肪性肝炎(NASH)的演变与独特的组织学特征有关。肝脏标本的病理评估经常受到观察者变异性的阻碍，并且诊断共识并不总是能够达成。我们研究了源于成人NASH的qFIBS技术是否适用于儿童NASH。资料与方法102例经肝活检证实为NASH的儿童患者，年龄18岁。连续切片行苏木精-伊红染色和Masson三色染色进行组织学评分，并进行二次谐波成像。以Nash CRN评分系统为参考标准，建立了qFIBS-纤维化、炎症、肝细胞气球和脂肪变性的自动化测量。结果定量FIBS与脂肪变性的相关性最好(r=0.001.84，P<0.0 1)，对不同程度的脂肪变性有较好的鉴别能力(AUROC0.90和0.98，敏感性0.71和0.93，特异性0.90和0.90)。QFIBS与纤维化的相关性良好(r=0.72，P<；0.001)，具有较高的AUROC值[qFibrosis(Auc)&Gt；0.85(0.85-0.95)]和区分不同纤维化阶段的能力。QFIBS与气球(r=0.38，P=0.028)和炎症(r=0.46，P=0.005)之间有较弱的相关性，但它可以区分不同级别的气球(AUROC0.73，敏感性0.36，特异性0.92)和炎症(AUROC0.77，敏感性0.83，特异性0.53)。结论成人NASH来源的qFIBS对儿童NASH最能区分脂肪变性和纤维化的不同组织学分级。
Background The evolution of pediatric non-alcoholic fatty liver disease (NAFLD) to non-alcoholic steatohepatitis (NASH) is associated with unique histological features. Pathological evaluation of liver specimen is often hindered by observer variability and diagnostic consensus is not always attainable. We investigated whether the qFIBS technique derived from adult NASH could be applied to pediatric NASH. Materials and Methods 102 pediatric patients (<18 years old) with liver biopsy-proven NASH were included. The liver biopsies were serially sectioned for hematoxylin-eosin and Masson trichrome staining for histological scoring, and for second harmonic generation (SHG) imaging. qFIBS-automated measure of fibrosis, inflammation, hepatocyte ballooning, and steatosis was estabilshed by using the NASH CRN scoring system as the reference standard. Results qFIBS showed the best correlation with steatosis (r = 0.84, P < 0.001); with ability to distinguish different grades of steatosis (AUROCs 0.90 and 0.98, sensitivity 0.71 and 0.93, and specificity 0.90 and 0.90). qFIBS correlation with fibrosis (r = 0.72, P < 0.001) was good with high AUROC values [qFibrosis (AUC) > 0.85 (0.85–0.95)] and ability to distinguish different stages of fibrosis. qFIBS showed weak correlation with ballooning (r = 0.38, P = 0.028) and inflammation (r = 0.46, P = 0.005); however, it could distinguish different grades of ballooning (AUROCs 0.73, sensitivity 0.36, and specificity 0.92) and inflammation (AUROCs 0.77, sensitivity 0.83, and specificity 0.53). Conclusion It was demonstrated that when qFIBS derived from adult NASH was performed on pediatric NASH, it could best distinguish the various histological grades of steatosis and fibrosis.
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影响因子：
29.4
作者：
Schwarz JM;Noworolski SM;Erkin-Cakmak A;Korn NJ;Wen MJ;Tai VW;Jones GM;Palii SP;Velasco-Alin M;Pan K;Patterson BW;Gugliucci A;Lustig RH;Mulligan K
通讯作者：
Mulligan K
影响因子：
5.1
作者：
Kleiner DE;Makhlouf HR
通讯作者：
Makhlouf HR
增强的肝纤维化评分与小儿非酒精脂肪肝疾病组织学以及对维生素E或二甲双胍的反应的关系。
DOI：
10.1016/j.jpeds.2021.08.012
发表时间：
2021-12
期刊：
The Journal of pediatrics
影响因子：
--
作者：
Gawrieh S;Harlow KE;Pike F;Yates KP;Wilson LA;Cummings OW;Rosenberg WM;Chalasani N;Molleston JP
通讯作者：
Molleston JP
影响因子：
4.2
作者：
Fukusato, T;Fukushima, J;Yoshimura, K
通讯作者：
Yoshimura, K
影响因子：
8
作者：
Sahota, Amandeep K.;Shapiro, Warren L.;Schwimmer, Jeffrey B.
通讯作者：
Schwimmer, Jeffrey B.