Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor.
Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor.
复制标题
通过 YtvA 光感受器通过光增强枯草芽孢杆菌中 sigma(B) 依赖性应激反应。
DOI：
--
复制
发表时间：
2007
影响因子：
1.2
通讯作者：
A. Nakamura
中科院分区：
文献类型：
--
作者：
N. Suzuki;N. Takaya;T. Hoshino;A. Nakamura
枯草芽孢杆菌的 YtvA 由光、氧或电压 (LOV) 结构域以及硫酸盐转运蛋白和抗 Sigma 拮抗剂 (STAS) 结构域组成，据报道其充当光感受器，通过 LOV 结构域感知光信号，就像植物蓝光受体、趋光素一样。同时，据报道，YtvA 作为受 sigma(B) 因子调节的应激反应基因表达的正调节因子。在这里，我们指出，像向光素一样，LOV结构域中保守的Cys残基是YtvA体外光传感所必需的，可能是通过光加合物的形成，并且YtvA通过其LOV结构域形成同二聚体，与光信号无关。我们还指出，当 ytvA 表达处于正常水平时，光本身不会触发 sigma(B) 激活，但由盐胁迫激活的 sigma(B) 活性的光增强仅在 ytvA 存在时发生。 LOV 结构域和 STAS 结构域中保守的 Cys 残基似乎分别负责光传感和向 sigma(B) 调节网络的信号传输。
YtvA of Bacillus subtilis consists of light, oxygen or voltage (LOV) domain and sulfate transporter and anti-sigma antagonist (STAS) domain, and was reported to act as a photoreceptor, sensing light signals through the LOV domain, like a plant blue light receptor, phototropin. At the same time, YtvA was reported to act as a positive regulator for stress responsive-gene expression regulated by sigma(B) factor. Here we indicate that, like phototropins, the conserved Cys residue among the LOV domains is required for light-sensing in YtvA in vitro, possibly by the photoadduct formation, and YtvA forms a homodimer via its LOV domain, independently to light signal. We also indicate that, when ytvA expression is in normal level, light itself does not trigger sigma(B) activation, but a photo-enhancement of sigma(B) activity, activated by salt stress, occurs only in the presence of ytvA. The conserved Cys residue in the LOV domain and the STAS domain seem to be responsible for light-sensing and signal-transmission to the sigma(B) regulatory network, respectively.
影响因子：
2.9
作者：
Crosson, S;Rajagopal, S;Moffat, K
通讯作者：
Moffat, K
影响因子：
3.5
作者：
HO, SN;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
影响因子：
5.6
作者：
Kim, TJ;Gaidenko, TA;Price, CW
通讯作者：
Price, CW