The Ly-6Chigh Monocyte Subpopulation Transports Listeria monocytogenes into the Brain during Systemic Infection of Mice1
The Ly-6Chigh Monocyte Subpopulation Transports Listeria monocytogenes into the Brain during Systemic Infection of Mice1
复制标题
Ly-6Chigh 单核细胞亚群在小鼠全身感染期间将单核细胞增生李斯特菌转运至大脑1
作者：
D. Drevets;M. J. Dillon;J. S. Schawang;N. Van Rooijen;J. Ehrchen;C. Sunderkötter;P. Leenen
单核细胞吞噬细胞可被胞内病原体用于在整个宿主中传播。在血液中，这些细胞通常被称为单核细胞。然而，血液单核细胞是一个异质群体，与微生物传播相关的白细胞的确切身份尚不清楚。本研究中报告的实验使用单核细胞增生李斯特菌感染的小鼠建立寄生血白细胞的表型，并测试它们在细胞内细菌全身传播中的作用。超过90%的与细菌相关的血液白细胞是CD 11b+单核细胞。新描述的单核细胞亚群的分析表明，大多数感染的细胞属于Ly-6Chigh单核细胞亚群，并且Ly-6Chigh和Ly-6Chigh低单核细胞每个细胞具有相似数量的细菌。有趣的是，用L.单核细胞增多症，这两个从吞噬体逃逸和细胞内复制，造成扩大的Ly-6Chigh子集。相比之下，这在用Δhly突变体感染后并不明显，Δ hly突变体既不逃避吞噬体也不在细胞内复制。重要的是，当从致死性感染小鼠的脑中分离出CD 11b+白细胞时，这些细胞中的88%被鉴定为Ly-6Chigh单核细胞。动力学分析显示，全身感染后2天，Ly-6Chigh单核细胞显着流入大脑。这与细菌入侵和脑巨噬细胞趋化蛋白-1基因表达的上调相一致。这些数据表明Ly-6Chigh单核细胞亚群转运L.单核细胞增多症进入大脑，并建立其作为体内特洛伊木马的作用。
Mononuclear phagocytes can be used by intracellular pathogens to disseminate throughout the host. In the bloodstream these cells are generically referred to as monocytes. However, blood monocytes are a heterogeneous population, and the exact identity of the leukocyte(s) relevant for microbial spreading is not known. Experiments reported in this study used Listeria monocytogenes-infected mice to establish the phenotype of parasitized blood leukocytes and to test their role in systemic dissemination of intracellular bacteria. More than 90% of the blood leukocytes that were associated with bacteria were CD11b+ mononuclear cells. Analysis of newly described monocyte subsets showed that most infected cells belonged to the Ly-6Chigh monocyte subset and that Ly-6Chigh and Ly-6Cneg-low monocytes harbored similar numbers of bacteria per cell. Interestingly, systemic infection with wild-type or ΔactA mutants of L. monocytogenes, both of which escape from phagosomes and replicate intracellularly, caused expansion of the Ly-6Chigh subset. In contrast, this was not evident after infection with Δhly mutants, which neither escape phagosomes nor replicate intracellularly. Importantly, when CD11b+ leukocytes were isolated from the brains of lethally infected mice, 88% of these cells were identified as Ly-6Chigh monocytes. Kinetic analysis showed a significant influx of Ly-6Chigh monocytes into the brain 2 days after systemic infection. This coincided with both bacterial invasion and up-regulation of brain macrophage chemoattractant protein-1 gene expression. These data indicate that the Ly-6Chigh monocyte subset transports L. monocytogenes into the brain and establish their role as Trojan horses in vivo.
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影响因子：
32.4
作者：
Serbina, NV;Salazar-Mather, TP;Pamer, EG
通讯作者：
Pamer, EG
病毒性脑炎感染各个阶段淋巴细胞归巢进入大脑的调节。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Irani,DN;Griffin,DE
通讯作者：
Griffin,DE
DOI：
10.1073/pnas.90.24.11890
发表时间：
1993-12-15
影响因子：
11.1
作者：
BRUNDAGE, RA;SMITH, GA;PORTNOY, DA
通讯作者：
PORTNOY, DA
影响因子：
2.2
作者：
Lagasse, E;Weissman, IL
通讯作者：
Weissman, IL