Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂).
Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂).
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红鲫鱼 (â) – 鲤鱼 (â) 二倍体和四倍体杂交种睾丸的 RNA Pol II CTD (S5) 定位和转录组分析
DOI：
10.3389/fgene.2021.717871
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发表时间：
2021
影响因子：
3.7
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Zhou Y;Zhu L;Sun Y;Zhang H;Wang J;Qin W;He W;Zhou L;Li Q;Zhao R;Luo K;Tang C;Zhang C;Liu S
多倍体在鱼类中自然发生;然而，这些物种的出现是一个偶然和渐进的过程，这使得很难追踪表型，基因型和基因表达调控的变化。红鲫（RCC）×鲤（CC）的异源四倍体杂种（4 nAT）是研究异源多倍体化后早期变化的良好模型。在本研究中，我们通过生物信息学分析，使用免疫荧光和RNA-seq数据，通过RNA Pol II CTD YSPTSPS（磷酸S5）定位，重点关注异源四倍体睾丸转录组活性位点的变化。结果表明，同一时期不同类型鱼（RCC、CC、2nF 1和4 nAT）的RNA Pol II CTD（S5）信号计数无显著差异，表明生殖细胞染色体上转录活性位点的数目不受染色体数目增加的影响。同样，RNA-seq分析表明，在染色体和基因组中10 kb区域的水平上，RCC、2nF 1和4 nAT中的高活性位点没有显著变化。这些发现表明，在四倍体起源的开始，睾丸中的4 nAT的活性转录组位点在基因组区域中与RCC和2nF 1相比是保守的。总之，4 nAT与RCC和2nF 1在基因组区域具有相似的基因表达模式，但表达水平显著不同。
Polyploidy occurs naturally in fish; however, the appearance of these species is an occasional and gradual process, which makes it difficult to trace the changes in phenotypes, genotypes, and regulation of gene expression. The allotetraploid hybrids (4nAT) of red crucian carp (RCC; ♀) × common carp (CC; ♂) generated from interspecies crossing are a good model to investigate the initial changes after allopolyploidization. In the present study, we focused on the changes in the active sites of the testicular transcriptome of the allotetraploid by localization of RNA Pol II CTD YSPTSPS (phospho S5) using immunofluorescence and RNA-seq data via bioinformatic analysis. The results showed that there was no significant difference in signal counts of the RNA Pol II CTD (S5) between the different types of fish at the same stages, including RCC, CC, 2nF1, and 4nAT, which means that the number of transcriptionally active sites on germ cell chromosomes was not affected by the increase in chromosome number. Similarly, RNA-seq analysis indicated that in the levels of chromosomes and 10-kb regions in the genome, there were no significant changes in the highly active sites in RCC, 2nF1, and 4nAT. These findings suggest that at the beginning of tetraploid origin, the active transcriptome site of 4nAT in the testis was conserved in the regions of the genome compared to that in RCC and 2nF1. In conclusion, 4nAT shared a similar gene expression model in the regions of the genome with RCC and 2nF1 with significantly different expression levels.
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影响因子：
5.6
作者：
Pelé A;Rousseau-Gueutin M;Chèvre AM
通讯作者：
Chèvre AM
影响因子：
4.2
作者：
Cowie P;Ross R;MacKenzie A
通讯作者：
MacKenzie A
金鱼与鲤鱼杂交的二倍体和四倍体后代的基因组不相容性
DOI：
10.1073/pnas.1512955113
发表时间：
2016-02-02
影响因子：
11.1
作者：
Liu, Shaojun;Luo, Jing;Zhang, Ya-Ping
通讯作者：
Zhang, Ya-Ping
影响因子：
4.5
作者：
Morgan, Michael D.;Patin, Etienne;Marioni, John C.
通讯作者：
Marioni, John C.
影响因子：
4
作者：
Rodriguez F;Arkhipova IR
通讯作者：
Arkhipova IR