Complete Genomic Analysis of a Kingdom-Crossing Klebsiella variicola Isolate.
Complete Genomic Analysis of a Kingdom-Crossing Klebsiella variicola Isolate.
复制标题
跨界的水痘克雷伯氏菌分离株的完整基因组分析
DOI：
10.3389/fmicb.2018.02428
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发表时间：
2018
影响因子：
5.2
通讯作者：
Gao Z
中科院分区：
文献类型：
--
作者：
Guo Y;Zhai Y;Zhang Z;Li D;Wang Z;Li J;He Z;Hu S;Kang Y;Gao Z
细菌分离株X39分离自中国北京的社区获得性肺炎患者。基于rpoB基因和平均核苷酸同源性数据构建的系统发育树证实X39菌株属于变异克雷伯氏菌。K. variicola X39含有1条环状染色体和9个质粒。与其它K. variicola分离物表明，K. variicola X39含有最独特的基因。在这些独特的基因中，许多是前噬菌体和转座酶。K. variicola X39和肺炎克雷伯氏菌F1。K. variicola X39与K. pneumoniae F1在腹腔感染模型中的作用。结果表明，K.变异克雷伯氏菌X39的毒力低于典型临床克雷伯氏菌。pneumoniae F1. K. variicola X39还含有一些与植物定殖、固氮和抗氧化胁迫有关的基因。GFP标记的K. variicola X39是一株能在玉米上定殖的内生细菌。我们得出结论，K。variicola X39是一个跨界菌株。
Bacterial isolate X39 was isolated from a community-acquired pneumonia patient in Beijing, China. A phylogenetic tree based on rpoB genes and average nucleotide identity data confirmed that isolate X39 belonged to Klebsiella variicola. The genome of K. variicola X39 contained one circular chromosome and nine plasmids. Comparative genomic analyses with other K. variicola isolates revealed that K. variicola X39 contained the most unique genes. Of these unique genes, many were prophages and transposases. Many virulence factors were shared between K. variicola X39 and Klebsiella pneumoniae F1. The pathogenicity of K. variicola X39 was compared with that of K. pneumoniae F1 in an abdominal infection model. The results indicated that K. variicola X39 was less virulent than typical clinical K. pneumoniae F1. The genome of K. variicola X39 also contained some genes involved in plant colonization, nitrogen fixation, and defense against oxidative stress. GFP-labeled K. variicola X39 could colonize maize as an endophytic bacterium. We concluded that K. variicola X39 was a kingdom-crossing strain.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
DOI：
10.1128/genomea.01015-15
发表时间：
2015-09-10
期刊：
Genome announcements
影响因子：
--
作者：
Martínez-Romero E;Silva-Sanchez J;Barrios H;Rodríguez-Medina N;Martínez-Barnetche J;Téllez-Sosa J;Gómez-Barreto RE;Garza-Ramos U
通讯作者：
Garza-Ramos U
影响因子：
14.9
作者：
Besemer, J;Lomsadze, A;Borodovsky, M
通讯作者：
Borodovsky, M
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
168.9
作者：
Martínez-Martínez, L;Pascual, A;Jacoby, GA
通讯作者：
Jacoby, GA