Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions.
Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions.
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DOI：
10.1002/anie.201006089
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发表时间：
2011-01-10
影响因子：
16.6
通讯作者：
Mathies, Richard A.
中科院分区：
文献类型：
--
作者：
Novak, Richard;Zeng, Yong;Shuga, Joe;Venugopalan, Gautham;Fletcher, Daniel A.;Smith, Martyn T.;Mathies, Richard A.
遗传分析，如聚合酶链反应（PCR），通常报告多个细胞或基因组DNA的混合物。因此，它们不能正确地表征细胞群的遗传异质性或检测单个细胞内不同突变的共同发生，而这是理解癌症的发生、进展和治疗的关键。[1,2]特别是，由于初始突变固有地发生在单细胞水平，因此单细胞分辨率的分析技术将极大地促进癌变的检测和表征。
Genetic assays, such as polymerase chain reaction (PCR), typically report on multiple cells or mixtures of genomic DNA. As a result, they cannot properly characterize the genetic heterogeneity of a cell population or detect the co-occurrence of different mutations within a single cell, which are key to understanding the development, progression, and treatment of cancers.[1, 2] In particular, since initial mutagenesis occurs inherently at the single-cell level, the detection and characterization of carcinogenesis will be dramatically facilitated by analytical techniques with single cell resolution.
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DOI：
10.1093/jncimonographs/lgn010
发表时间：
2008-01-01
期刊：
Journal of the National Cancer Institute. Monographs
影响因子：
--
作者：
McHale, Cliona M;Lan, Qing;Smith, Martyn T
通讯作者：
Smith, Martyn T
影响因子：
11.5
作者：
d'Amore, Francesco;Chan, Eric;Dave, Bhavana J.
通讯作者：
Dave, Bhavana J.
影响因子：
4.3
作者：
Huan Huang;Ying Bu;Guo-Hua Zhou
通讯作者：
Guo-Hua Zhou
影响因子：
7
作者：
Navin, Nicholas;Krasnitz, Alexander;Wigler, Michael
通讯作者：
Wigler, Michael
影响因子：
7.4
作者：
Kumaresan, Palani;Yang, Chaoyong James;Mathies, Richard A.
通讯作者：
Mathies, Richard A.