Ultrastructural localization of dystropin in human muscle by using gold immunolabelling
Ultrastructural localization of dystropin in human muscle by using gold immunolabelling
复制标题
使用金免疫标记对人体肌肉中抗肌萎缩蛋白进行超微结构定位
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
J. Harris
中科院分区：
文献类型：
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作者：
M. Cullen;J. Walsh;L. Nicholson;J. Harris
免疫标记与5 nm的金探针被用来本地化肌营养不良蛋白在超微结构水平在人类肌肉。第一抗体是单克隆的，针对来自肌营养不良蛋白的杆结构域的区段（氨基酸1181-1388）产生。抗体（Dy 4/6D 3）对肌营养不良蛋白具有特异性，与mdx小鼠肌肉或抗体识别分子部分基因缺失患者的任何蛋白均无免疫反应性（Nicholson et al. 1989 a; England et al. 1990）。使用这种抗体，标记几乎完全局限于肌肉纤维周围的狭窄的75 nm边缘。从金探针到质膜的细胞质面的距离和金探针之间的距离（在平行于质膜的平面中的最近邻）的直方图分别显示在约15 nm和120 nm处的模式。探头在肌肉纵、横切面上的分布相同。这些观察结果表明，肌营养不良蛋白分子的杆部分通常被安排接近质膜的细胞质面和分子形成一个相互连接的网络。标记与横管系统无关。
Immunolabelling with a 5 nm gold probe was used to localize dystrophin at the ultrastructural level in human muscle. The primary antibody was monoclonal, raised against a segment (amino acids 1181-1388) from the rod domain of dystrophin. The antibody (Dy4/6D3) is specific for dystrophin and shows no immunoreactivity with any protein from mdx mouse muscle or from patients with a gene deletion spanning part of the molecule recognized by the antibody (Nicholson et al. 1989a; England et al. 1990). Using this antibody, labelling was almost entirely confined to a narrow 75 nm rim at the periphery of the muscle fibres. Histograms of the distance from the gold probe to the cytoplasmic face of the plasma membrane and of the distance between gold probes (nearest neighbour in a plane parallel with the plasma membrane) displayed modes at approximately 15 nm and 120 nm, respectively. The distribution of the probe was the same in longitudinal and transverse sections of the muscle. These observations suggest that the rod portion of the dystrophin molecule is normally arranged close to the cytoplasmic face of the plasma membrane and that the molecules form an interconnecting network. Labelling was not associated with the transverse tubular system.
细胞分级分离研究表明肌营养不良蛋白是骨骼肌表面膜的蛋白质。
DOI：
10.1042/bj2580837
发表时间：
1989
期刊：
The Biochemical journal
影响因子：
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作者：
Salviati,G;Betto,R;Ceoldo,S;Biasia,E;Bonilla,E;Miranda,AF;Dimauro,S
通讯作者：
Dimauro,S