SIVA1 Regulates the Stability of Single-Stranded DNA-Binding Protein 3 Isoforms
SIVA1 Regulates the Stability of Single-Stranded DNA-Binding Protein 3 Isoforms
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SIVA1 调节单链 DNA 结合蛋白 3 亚型的稳定性
DOI：
10.1134/s0026893318050163
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发表时间：
2018-09
影响因子：
1.2
通讯作者：
Mo X
中科院分区：
文献类型：
--
作者：
Yin Z;Zhang K;Peng X;Jiang Z;Yuan W;Wang Y;Li Y;Ye X;Dong Y;Wan Y;Ni B;Zhu P;Fan X;Wu X;Mo X
LIM-HD转录复合体的组装在早期神经元发育中起重要作用。LIM-HD的稳定性由单链结合蛋白3（SSBP 3）通过保护其免受蛋白酶体降解的级联机制控制。SSBP 3的表达水平必须精确调控。尽管SSBP 3水平的降低与几种疾病有关，但SSBP 3下调的机制以及SSBP 3本身是否受到蛋白酶体降解的影响仍然是未知的。从人脑cDNA文库中克隆了SSBP 3基因的两个高度保守的转录本SSBP 3a和SSBP 3c。通过RT-PCR，我们发现Ssbp 3c在胚胎和成年小鼠脑中持续表达，而Ssbp 3a仅限于胚胎脑组织。通过co-IP和GST pulldown分析，我们确定SIVA 1为一种新的SSBP 3结合因子。在泛素化测定中，我们发现SIVA 1增强SSBP 3的泛素化并调节其丰度。我们的研究结果首次揭示了SSBP 3的蛋白酶体降解，并为LIM-HD多蛋白复合物的分解提供了SIVA 1-SSBP 3依赖性机制的理论基础。
The assembly of LIM-homeodomain (LIM-HD) transcriptional complex plays important roles in early neuronal development. The stability of LIM-HD is controlled by single-strand binding protein 3 (SSBP3) via a cascade mechanism protecting it from proteasomal degradation. The expression level of SSBP3 has to be precisely regulated. Although a decrease of SSBP3 level is associated with several diseases, the mechanism of SSBP3 downregulation and whether SSBP3 itself is subject to proteasomal degradation remain largely unknown. Two strongly conserved transcripts of the SSBP3 gene, SSBP3a and SSBP3c, were cloned from a human brain cDNA library. By RT-PCR, we show that Ssbp3c is continuously expressed in both embryonic and adult mouse brain, whereas Ssbp3a is restricted to embryonic brain tissue. By co-IP and GST pulldown assays, we identified SIVA1 as a novel SSBP3-binding factor. In a ubiquitination assay, we show that SIVA1 enhances the ubiquitination of SSBP3 and regulates its abundance. Our findings reveal the proteasomal degradation of SSBP3 for the first time and provide a rationale for an SIVA1-SSBP3-dependent mechanism for the disassembly of LIM-HD multiprotein complexes.
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影响因子：
3.7
作者：
Li W;Bengtson MH;Ulbrich A;Matsuda A;Reddy VA;Orth A;Chanda SK;Batalov S;Joazeiro CA
通讯作者：
Joazeiro CA
DOI：
10.1073/pnas.212532399
发表时间：
2002-10-29
影响因子：
11.1
作者：
Chen, L;Segal, D;Westphal, H
通讯作者：
Westphal, H
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影响因子：
3.8
作者：
Fan, Xiongwei;Yang, Qing;Wu, Xiushan
通讯作者：
Wu, Xiushan
影响因子：
4.6
作者：
Hiratani, I;Yamamoto, N;Taira, M
通讯作者：
Taira, M
影响因子：
4
作者：
Resch, Ulrike;Schichl, Yvonne M.;de Martin, Rainer
通讯作者：
de Martin, Rainer