Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery.
Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery.
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DOI：
10.3390/microorganisms9071376
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发表时间：
2021-06-24
期刊：
影响因子：
4.5
通讯作者：
Stevens DC
中科院分区：
文献类型：
--
作者：
Ahearne A;Albataineh H;Dowd SE;Stevens DC
新型粘细菌的发现已开始揭示粘球菌科内潜在的巨大系统发育多样性，并带来了粘细菌分类的更新方法。传统方法侧重于形态学、16S 基因序列和生物化学，而包括比较基因组学在内的现代方法为粘细菌分类学提供了更全面的评估。在此，我们利用长读长基因组测序对两种先前分类为Archangium primigenium和Chondrococcus macrosporus的粘细菌，以及本研究中新分离的四种环境粘细菌进行分析。比较基因组学的平均核苷酸同一性和数字 DNA-DNA 杂交评分表明，之前被分类为 A. primigenium 的为 Melittangium 属的新成员，C. macrosporus 为珊瑚球菌属的潜在新成员，与运动珊瑚球菌高度相似，四种分离的粘细菌包括另一种新的珊瑚球菌属物种、一种新的 Pyxidicoccus 物种、一种珊瑚球菌菌株exiguus，以及与粘球菌 stipitatus 高度相似的潜在新型粘球菌属物种。我们评估了每种已测序粘细菌的生物合成潜力，并建议生物合成途径的属级保守性支持我们的初步分类学分配。总而言之，我们认为长读长基因组测序有利于粘细菌的分类，并提高了生物合成潜力的确定，从而优先考虑天然产物的发现。
Discoveries of novel myxobacteria have started to unveil the potentially vast phylogenetic diversity within the family Myxococcaceae and have brought about an updated approach to myxobacterial classification. While traditional approaches focused on morphology, 16S gene sequences, and biochemistry, modern methods including comparative genomics have provided a more thorough assessment of myxobacterial taxonomy. Herein, we utilize long-read genome sequencing for two myxobacteria previously classified as Archangium primigenium and Chondrococcus macrosporus, as well as four environmental myxobacteria newly isolated for this study. Average nucleotide identity and digital DNA–DNA hybridization scores from comparative genomics suggest previously classified as A. primigenium to instead be a novel member of the genus Melittangium, C. macrosporus to be a potentially novel member of the genus Corallococcus with high similarity to Corallococcus exercitus, and the four isolated myxobacteria to include another novel Corallococcus species, a novel Pyxidicoccus species, a strain of Corallococcus exiguus, and a potentially novel Myxococcus species with high similarity to Myxococcus stipitatus. We assess the biosynthetic potential of each sequenced myxobacterium and suggest that genus-level conservation of biosynthetic pathways support our preliminary taxonomic assignment. Altogether, we suggest that long-read genome sequencing benefits the classification of myxobacteria and improves determination of biosynthetic potential for prioritization of natural product discovery.
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影响因子：
2.7
作者：
Garrison NL;Rodriguez J;Agnarsson I;Coddington JA;Griswold CE;Hamilton CA;Hedin M;Kocot KM;Ledford JM;Bond JE
通讯作者：
Bond JE
影响因子：
3.2
作者：
Erol, Oezlem;Schaeberle, Till F.;Koenig, Gabriele M.
通讯作者：
Koenig, Gabriele M.
DOI：
10.1111/j.1432-1033.1995.238_1.x
发表时间：
1995-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BOTELLA, JA;MURILLO, FJ;RUIZVAZQUEZ, R
通讯作者：
RUIZVAZQUEZ, R
影响因子：
3.3
作者：
Baltz, Richard H.
通讯作者：
Baltz, Richard H.
影响因子：
14.9
作者：
Blin K;Shaw S;Kautsar SA;Medema MH;Weber T
通讯作者：
Weber T