Metabolic Profiling of Amino Acids Associated with Mortality in Patients with Acute Paraquat Poisoning.
Metabolic Profiling of Amino Acids Associated with Mortality in Patients with Acute Paraquat Poisoning.
复制标题
与急性百草枯中毒患者死亡率相关的氨基酸代谢谱
DOI：
10.12659/msm.905843
复制
发表时间：
2018-03-07
期刊：
影响因子：
--
通讯作者：
Peng A
中科院分区：
文献类型：
--
作者：
Wan X;Zhou C;Kang X;Hu D;Xue W;Li X;Bao H;Peng A
背景 在亚洲和太平洋国家的许多地区，百草枯是摄入后致命中毒的主要原因。然而，评估患者死亡率的最佳预后指标尚未明确建立。急性百草枯中毒后，许多氨基酸 (AA) 在代谢途径中表达异常。然而，人类百草枯中毒后 AA 代谢物水平的变化仍然未知。材料/方法 在本研究中，纳入了 40 名患者，其中 16 名存活，24 名死亡。采用代谢组学方法评估百草枯中毒后血浆中 AA 代谢物的变化及其潜在的预后价值。进行了基于代谢物鉴定的质谱分析（MS）。结果 死亡患者血浆中 25 种 AA 水平表达异常。其中，肌酐、吲哚乳酸盐和3-(4-羟苯基)乳酸盐被发现与百草枯死亡预测高度相关。值得注意的是，非幸存者组中这 3 种 AA 代谢物的强度水平显着高于幸存者组。此外，我们还检查了受试者工作特征 (ROC) 曲线以进行临床验证。 ROC 结果显示，3-(4-羟苯基)乳酸盐的 AUC 最高，为 0.84，而吲哚乳酸盐和肌酐的 AUC 分别为 0.75 和 0.83，表明它们可用于预测临床结果（尽管这种方法实施起来成本昂贵）。结论 AA 水平的代谢分析可以成为确定百草枯中毒早期高精度预后有效指标的可靠工具。
Background Paraquat is a major cause of fatal poisoning after ingestion in many parts of Asia and the Pacific nations. However, optimal prognostic indicators to evaluate patient mortality have not been unequivocally established. Following acute paraquat poisoning, a number of amino acids (AA), are abnormally expressed in metabolic pathways. However, the alterations in AA metabolite levels after paraquat poisoning remain unknown in humans. Material/Methods In the present study, 40 patients were enrolled, of whom 16 survived and 24 died. A metabolomics approach was used to assess changes in AA metabolites in plasma and its potential prognostic value following paraquat poisoning. Mass spectrometry (MS) based on metabolite identification was conducted. Results Twenty-five AA levels in plasma were abnormally expressed in non-survivor patients. Among them, creatinine, indolelactate, and 3-(4-hydroxyphenyl)lactate were found to be highly correlated with paraquat death prediction. It was noted that the intensity levels of these 3 AA metabolites in the non-survivor group were substantially higher than in the survivor group. Furthermore, we examined receiver operating characteristic (ROC) curves for clinical validation. ROC results showed that 3-(4-hydroxyphenyl)lactate had the highest AUC of 0.84, while indolelactate and creatinine had AUCs of 0.75 and 0.83, respectively, suggesting that they can be used to predict the clinical outcome (although this methodology is expensive to implement). Conclusions Metabolic profiling of AA levels could be a reliable tool to identify effective indicators for the early high precision prognosis of paraquat poisoning.
登录
查看更多内容
影响因子：
3.7
作者：
Weng CH;Hu CC;Lin JL;Lin-Tan DT;Huang WH;Hsu CW;Yen TH
通讯作者：
Yen TH
影响因子：
4.6
作者：
Li Y;Wang M;Gao Y;Yang W;Xu Q;Eddleston M;Li L;Yu X
通讯作者：
Yu X
DOI：
10.1016/j.metabol.2016.12.010
发表时间：
2017-04
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Jonker R;Deutz NE;Erbland ML;Anderson PJ;Engelen MP
通讯作者：
Engelen MP
影响因子：
8.9
作者：
Korashy, Hesham M.;Attafi, Ibraheem M.;Al-Ghadeer, Abdul Rahman M.
通讯作者：
Al-Ghadeer, Abdul Rahman M.
影响因子：
2.8
作者：
Lee, Jun Ho;Hwang, Seong Youn;Kim, Yong Hwan
通讯作者：
Kim, Yong Hwan