Prevalence of genetic differences in phosphorylcholine expression between nontypeable Haemophilus influenzae and Haemophilus haemolyticus.
Prevalence of genetic differences in phosphorylcholine expression between nontypeable Haemophilus influenzae and Haemophilus haemolyticus.
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DOI：
10.1186/1471-2180-10-286
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发表时间：
2010-11-12
期刊：
影响因子：
4.2
通讯作者：
Gilsdorf JR
中科院分区：
文献类型：
--
作者：
McCrea KW;Xie J;Marrs CF;Gilsdorf JR
虽然不可分型流感嗜血杆菌和溶血嗜血杆菌是密切相关的人类共生菌，但溶血嗜血杆菌是非致病性的，而NT流感嗜血杆菌是呼吸道感染的重要原因。低脂寡糖（LOS）的相位可变磷酸胆碱（ChoP）修饰是一种NT流感嗜血杆菌毒力因子，矛盾的是，它也可能通过结合c反应蛋白（CRP）促进补体活化。已知CRP更多地与位于LOS远端的ChoP结合，而不是近端ChoP，并且ChoP在LOS中的位置由lic1位点中存在的特定licD等位基因（这里指定为licDI， licDIII和licDIV）决定。lic1位点包含licA- licd基因，并且ChoP-宿主相互作用也可能受到第二个lic1位点的影响，该位点允许在同一菌株中进行双重ChoP替换，或者受到licA基因四核苷酸重复数（5'-CAAT-3'）的影响，该重复数反映了相位变化突变率。利用斑点杂交技术，88株NT流感嗜血杆菌中有92%和109株溶血嗜血杆菌中有42.6%具有lic1位点。8%的NT流感嗜血杆菌株和溶血嗜血杆菌株都没有lic1的双拷贝。licDIII和licDIV等位基因在流感嗜血杆菌和溶血嗜血杆菌中分布相似（18-22%），而licDI等位基因在45.5%的流感嗜血杆菌中存在，而在溶血嗜血杆菌中不到1% （P < 0.0001）。NT流感嗜血杆菌在licA中平均有26.8个四核苷酸重复，而在溶血嗜血杆菌中平均有14.8个重复（P < 0.05）。此外，具有licDIII等位基因的流感NT H.菌株与具有其他licD等位基因的流感NT H.菌株相比，具有licDIII等位基因的流感NT H.菌株重复次数增加（P < 0.05）。这些数据表明，流感嗜血杆菌和溶血嗜血杆菌之间存在ChoP表达的遗传相似性和差异，并加强了在群体水平上，这些差异可能在一定程度上为流感嗜血杆菌的毒力提供了优势的假设。
Although non-typeable (NT) Haemophilus influenzae and Haemophilus haemolyticus are closely related human commensals, H. haemolyticus is non-pathogenic while NT H. influenzae is an important cause of respiratory tract infections. Phase-variable phosphorylcholine (ChoP) modification of lipooligosaccharide (LOS) is a NT H. influenzae virulence factor that, paradoxically, may also promote complement activation by binding C-reactive protein (CRP). CRP is known to bind more to ChoP positioned distally than proximally in LOS, and the position of ChoP within LOS is dictated by specific licD alleles (designated here as licDI, licDIII, and licDIV) that are present in a lic1 locus. The lic1 locus contains the licA-licD genes, and ChoP-host interactions may also be influenced by a second lic1 locus that allows for dual ChoP substitutions in the same strain, or by the number of licA gene tetranucleotide repeats (5'-CAAT-3') that reflect phase-variation mutation rates. Using dot-blot hybridization, 92% of 88 NT H. influenzae and 42.6% of 109 H. haemolyticus strains possessed a lic1 locus. Eight percent of NT H. influenzae and none of the H. haemolyticus strains possessed dual copies of lic1. The licDIII and licDIV gene alleles were distributed similarly (18-22%) among the NT H. influenzae and H. haemolyticus strains while licDI alleles were present in 45.5% of NT H. influenzae but in less than 1% of H. haemolyticus strains (P < .0001). NT H. influenzae had an average of 26.8 tetranucleotide repeats in licA compared to14.8 repeats in H. haemolyticus (P < .05). In addition, NT H. influenzae strains that possessed a licDIII allele had increased numbers of repeats compared to NT H. influenzae with other licD alleles (P < .05). These data demonstrate that genetic similarities and differences of ChoP expression exist between NT H. influenzae and H. haemolyticus and strengthen the hypothesis that, at the population level, these differences may, in part, provide an advantage in the virulence of NT H. influenzae.
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影响因子：
12.3
作者：
Hogg JS;Hu FZ;Janto B;Boissy R;Hayes J;Keefe R;Post JC;Ehrlich GD
通讯作者：
Ehrlich GD
影响因子：
2.9
作者：
LEON, MA;YOUNG, NM
通讯作者：
YOUNG, NM
DOI：
10.1007/bf02014895
发表时间：
1984-01-01
影响因子：
4.5
作者：
KUKLINSKA, D;KILIAN, M
通讯作者：
KILIAN, M
影响因子：
3.6
作者：
Lysenko, E;Richards, JC;Weiser, JN
通讯作者：
Weiser, JN
影响因子：
6.4
作者：
Goldenberg, HB;McCool, TL;Weiser, JN
通讯作者：
Weiser, JN