Immunocompetent cells in rat periodontal ligament and their recruitment incident to experimental orthodontic tooth movement.
Immunocompetent cells in rat periodontal ligament and their recruitment incident to experimental orthodontic tooth movement.
复制标题
大鼠牙周膜中的免疫活性细胞及其在实验性正畸牙齿移动过程中的募集。
DOI：
10.1111/j.1600-0722.1997.tb00178.x
复制
发表时间：
1997
影响因子：
1.9
通讯作者：
R. Jonsson
中科院分区：
文献类型：
--
作者：
V. Vandevska;I. Kvinnsland;S. Kvinnsland;R. Jonsson
本研究的目的是评估免疫活性细胞在正常大鼠牙周膜（PDL）的数量和分布，并量化其招聘事件实验牙齿移动。27只幼鼠分别用正畸矫治器近中移动右侧上颌第一磨牙1、3、7和14天。4只动物作为未处理对照。免疫组织化学程序进行了交替系列冷冻切片，并对CD11b（巨噬细胞，树突状细胞），CD43（淋巴细胞，多态性），CD4（辅助T淋巴细胞），和II类MHC分子的单克隆抗体被使用。对照组牙周膜中CD11b+、CD43+和II类分子表达细胞数最多，而CD4+和CD43+细胞数较少，实验移动后牙周膜中CD11b+、CD43+和II类分子表达细胞数较对侧和对照组显著增加。而CD4+细胞无明显增加。在玻璃样变组织周围、牙本质与细胞牙骨质之间、Malassez上皮细胞附近可见CD 11b+和表达II类分子的细胞。结论：正常大鼠牙周膜内巨噬细胞和树突状细胞数量较多，淋巴细胞和粒细胞数量较少。此外，实验性牙齿移动导致属于单核吞噬细胞系统的细胞的显著募集，但对大鼠PDL中的淋巴细胞和粒细胞的数量没有显著影响。
The aims of this study were to evaluate the number and distribution of immunocompetent cells in normal rat periodontal ligament (PDL) and to quantify their recruitment incident to experimental tooth movement. 27 young animals had the 1st right maxillary molar moved mesially by an orthodontic appliance for 1, 3, 7 and 14 days, respectively. 4 animals served as untreated controls. An immunohistochemical procedure was carried out on alternate serial cryostat sections, and monoclonal antibodies against CD11b (macrophages, dendritic cells), CD43 (lymphocytes, polymorphs), CD4 (helper T-lymphocytes), and class II MHC molecules were used. Mean counts of the immunolabeled cells in the control group showed the highest number of CD11b+ and class II molecule expressing cells, while CD4+ and CD43+ cells were scarcely found. Significant increase in the number of CD11b+, CD43+ cells and class II molecules was found in the PDL of the experimentally moved 1st molars compared with the contralateral side and the control group, while CD4+ cells showed no significant increase. CD11b+ and cells expressing class II molecules were found around hyalinized tissue, between dentin and cellular cementum and close to Malassez' epithelial cells. In conclusion, normal rat PDL has high number of macrophage and dendritic-like cells, but few lymphocytes and granulocytes. Furthermore, experimental tooth movement leads to significant recruitment of cells belonging to the mononuclear phagocytic system, but has no significant effect on the number of lymphocytes and granulocytes in the rat PDL.
影响因子：
4.4
作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
通讯作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
DOI：
10.1073/pnas.90.7.3038
发表时间：
1993-04-01
影响因子：
11.1
作者：
INABA, K;INABA, M;STEINMAN, RM
通讯作者：
STEINMAN, RM
重组白细胞介素 1、白细胞介素 2 和干扰素-γ 对体外骨吸收的作用。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gowen,M;Mundy,GR
通讯作者：
Mundy,GR