Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase
Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase
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铜绿假单胞菌乙酰辅酶A羧化酶生物素羧基载体蛋白和生物素羧化酶蛋白编码基因的组织结构和核苷酸序列
DOI：
10.1128/jb.175.21.6881-6889.1993
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发表时间：
1993
影响因子：
3.2
通讯作者：
V C Knauf
中科院分区：
文献类型：
--
作者：
E A Best;V C Knauf
通过克隆和表征铜绿假单胞菌乙酰辅酶A羧化酶（ACC）的DNA片段，研究了ACC的遗传组织。DNA测序和RNA印迹杂交研究表明，编码ACC生物素羧基载体蛋白（BCCP）亚基的铜绿假单胞菌accB（fabE）同源物，是2基因操纵子的一部分，包括accC（fabG），ACC的生物素羧化酶亚基的结构基因。大肠杆菌accA和accD编码ACC羧基转移酶的α和β亚基。coli accA和accD.所提供的数据表明铜绿假单胞菌accA和accD同源物不直接位于铜绿假单胞菌accBC操纵子的上游或下游。与E.大肠杆菌中，其中BCCP是唯一的生物素化蛋白，铜绿假单胞菌被发现含有至少三个生物素化蛋白。
The genetic organization of the Pseudomonas aeruginosa acetyl coenzyme A carboxylase (ACC) was investigated by cloning and characterizing a P. aeruginosa DNA fragment that complements an Escherichia coli strain with a conditional lethal mutation affecting the biotin carboxyl carrier protein (BCCP) subunit of ACC. DNA sequencing and RNA blot hybridization studies indicated that the P. aeruginosa accB (fabE) homolog, which encodes BCCP, is part of a 2-gene operon that includes accC (fabG), the structural gene for the biotin carboxylase subunit of ACC. P. aeruginosa homologs of the E. coli accA and accD, encoding the alpha and beta subunits of the ACC carboxyltransferase, were identified by hybridization of P. aeruginosa genomic DNA with the E. coli accA and accD. Data are presented which suggest that P. aeruginosa accA and accD homologs are not located either immediately upstream or downstream of the P. aeruginosa accBC operon. In contrast to E. coli, where BCCP is the only biotinylated protein, P. aeruginosa was found to contain at least three biotinylated proteins.
影响因子：
2.6
作者：
DITTA, G;SCHMIDHAUSER, T;HELINSKI, DR
通讯作者：
HELINSKI, DR
含有遗传连锁的交替抗生素抗性元件的菌株集合，用于大肠杆菌的遗传图谱。
DOI：
10.1128/mr.53.1.1-24.1989
发表时间：
1989
期刊：
Microbiological reviews
影响因子：
--
作者：
Singer,M;Baker,TA;Schnitzler,G;Deischel,SM;Goel,M;Dove,W;Jaacks,KJ;Grossman,AD;Erickson,JW;Gross,CA
通讯作者：
Gross,CA
影响因子：
2.6
作者：
KNAUF, VC;NESTER, EW
通讯作者：
NESTER, EW