Revisiting the sialome of the cat flea Ctenocephalides felis.
Revisiting the sialome of the cat flea Ctenocephalides felis.
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DOI：
10.1371/journal.pone.0279070
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Ribeiro, Jose M. C.
中科院分区：
文献类型：
--
作者：
Lu, Stephen;Danchenko, Monika;Macaluso, Kevin R.;Ribeiro, Jose M. C.
在节肢动物的进化过程中，有几次吸血行为独立出现。唾液腺和唾液成分及其药理活性的调查得出的结论是，吸血节肢动物进化出一种独特的唾液混合物，可以干扰宿主的防御反应，从而促进血液采集和病原体传播。猫栉首蚤（Ctenocephalides felis）是包括伤寒立克次体、猫立克次体和巴尔通体属（Bartonella spp.）因此，从医学和兽医学的角度来看，它是一种重要的昆虫物种。以前，基于Sanger的成年C.发表了1，840个表达序列标签（EST），组装成896个重叠群。在这里，我们提供了一个更深入的了解C。使用基于Illumina的测序方法测定猫唾液腺组成。在当前的数据集中，我们报告了8，892个编码序列（CDS），分为27个功能类别，它们是从42，754，615个读取中组装的。此外，我们将我们的RNAseq数据与使用翻译的转录本作为参考的质谱分析配对，证实了猫蚤唾液腺匀浆中存在几个推定的分泌蛋白家族。转录组学和蛋白质组学方法均证实，FS-H样蛋白和缺乏推定催化残基的酸性磷酸酶是C.可能与血液采集有关。我们还报告了几个新的序列类似于腺苷三磷酸双磷酸酶，气味结合蛋白，抗原5，胆碱酯酶，蛋白酶和蛋白酶抑制剂，除了推定的新序列，提出低或没有序列同一性，以前存放的序列。总之，这些数据代表了对C中存在的药理活性的鉴定和表征的扩展参考。猫的唾液腺
The hematophagous behaviour emerged independently in several instances during arthropod evolution. Survey of salivary gland and saliva composition and its pharmacological activity led to the conclusion that blood-feeding arthropods evolved a distinct salivary mixture that can interfere with host defensive response, thus facilitating blood acquisition and pathogen transmission. The cat flea, Ctenocephalides felis, is the major vector of several pathogens, including Rickettsia typhi, Rickettsia felis and Bartonella spp. and therefore, represents an important insect species from the medical and veterinary perspectives. Previously, a Sanger-based sialome of adult C. felis female salivary glands was published and reported 1,840 expressing sequence tags (ESTs) which were assembled into 896 contigs. Here, we provide a deeper insight into C. felis salivary gland composition using an Illumina-based sequencing approach. In the current dataset, we report 8,892 coding sequences (CDS) classified into 27 functional classes, which were assembled from 42,754,615 reads. Moreover, we paired our RNAseq data with a mass spectrometry analysis using the translated transcripts as a reference, confirming the presence of several putative secreted protein families in the cat flea salivary gland homogenates. Both transcriptomic and proteomic approaches confirmed that FS-H-like proteins and acid phosphatases lacking their putative catalytic residues are the two most abundant salivary proteins families of C. felis and are potentially related to blood acquisition. We also report several novel sequences similar to apyrases, odorant binding proteins, antigen 5, cholinesterases, proteases, and proteases inhibitors, in addition to putative novel sequences that presented low or no sequence identity to previously deposited sequences. Together, the data represents an extended reference for the identification and characterization of the pharmacological activity present in C. felis salivary glands.
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影响因子：
9.8
作者：
Alvarenga PH;Francischetti IM;Calvo E;Sá-Nunes A;Ribeiro JM;Andersen JF
通讯作者：
Andersen JF
DOI：
10.1016/b978-0-12-814043-7.00010-8
发表时间：
2019-01-01
期刊：
MEDICAL AND VETERINARY ENTOMOLOGY, 3RD EDITION
影响因子：
--
作者：
Durden, Lance A.;Hinkle, Nancy C.
通讯作者：
Hinkle, Nancy C.
影响因子：
5.4
作者：
Driscoll, Timothy P.;Verhoeve, Victoria, I;Azad, Abdu F.
通讯作者：
Azad, Abdu F.
影响因子：
3.4
作者：
Eng, Jimmy K.;Jahan, Tahmina A.;Hoopmann, Michael R.
通讯作者：
Hoopmann, Michael R.
影响因子：
56.9
作者：
BONTEMS, F;ROUMESTAND, C;TOMA, F
通讯作者：
TOMA, F