Ambroxol Inhibits Na+ Absorption by Canine Airway Epithelial Cells in Culture
Ambroxol Inhibits Na+ Absorption by Canine Airway Epithelial Cells in Culture
复制标题
氨溴索抑制培养物中犬气道上皮细胞对 Na 的吸收
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
T. Takizawa
中科院分区：
文献类型：
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作者：
J. Tamaoki;A. Chiyotani;F. Yamauchi;S. Takeuchi;T. Takizawa
摘要：为了研究氨溴索对气道粘膜离子转运功能的影响，我们在体外测试了短路条件下培养的犬气管上皮的生物电特性。将氨溴索添加到粘膜下，而不是添加到Ussing室的粘膜溶液中，可以降低短路电流、上皮间电位差和细胞电导。氨溴索诱导的短路电流的降低不受布美他尼或二苯胺- 2 -羧酸盐的影响，但用阿米洛利预处理细胞可以消除。这些结果表明氨溴索可能选择性地抑制气道上皮对Na+的吸收，从而增加气道表面液体中的水分组成，降低黏液粘度。
Abstract— To study the effect of ambroxol on ion transport functions of airway mucosa, we measured bioelectric properties of canine cultured tracheal epithelium under short‐circuit conditions in‐vitro. Addition of ambroxol to the submucosal but not to the mucosal solution in an Ussing chamber decreased short‐circuit current, transepithelial potential difference and cell conductance. The ambroxol‐induced decrease in short‐circuit current was not affected by bumetanide or diphenylamine‐2‐carboxylate, but it was abolished by pretreatment of cells with amiloride. These results suggest that ambroxol may selectively inhibit Na+ absorption by airway epithelium, thereby increasing water composition in airway surface fluid and reducing mucus viscosity.
“袢”利尿剂对狗气管上皮离子转运的影响。
DOI：
10.1152/ajpcell.1983.245.5.c388
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
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作者：
Widdicombe,JH;Nathanson,IT;Highland,E
通讯作者：
Highland,E