Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival.
Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival.
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DOI：
10.3390/biom12111584
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发表时间：
2022-10-28
期刊：
影响因子：
5.5
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中科院分区：
文献类型：
--
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低温（冷）保存是成功的细胞和组织移植的限制因素，其中细胞肿胀（水肿）通常发展，损害细胞功能。威斯康星州大学（UW）溶液是一种标准的冷保存溶液，其含有抑制低氧诱导的细胞肿胀的有效成分。抗冻蛋白（Antifreeze Proteins，AFP）存在于许多冷适应生物体中，可以防止生物体的冷害。本文首次研究了加拿大石斛甲胎蛋白（DAFP-1）对胰岛β细胞保存的影响。低至500 μg/mL，DAFP-1在UW溶液中4 °C保存长达三天期间显著最小化INS-1细胞肿胀和随后的细胞死亡。然而，这种显着的细胞保护没有观察到的AFP从黄粉虫（TmAFP），结构同源DAFP-1，但缺乏精氨酸，在相同的水平。DAFP-1的细胞保护作用进一步用UW溶液中分离的大鼠胰岛中的原代β细胞验证。亚毫克水平的DAFP-1的UW溶液的补充显着增加胰岛质量恢复后，三天的冷保存，然后复温。在分子水平上讨论了DAFP-1在UW溶液中的保护作用。结果表明DAFP-1在延长的冷保存期间提高细胞存活的潜力。
Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic β-cells preservation were first investigated. As low as 500 µg/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 °C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary β-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation.
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