DNA methylation suppresses chitin degradation and promotes the wing development by inhibiting Bmara-mediated chitinase expression in the silkworm, Bombyx mori
DNA methylation suppresses chitin degradation and promotes the wing development by inhibiting Bmara-mediated chitinase expression in the silkworm, Bombyx mori
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DNA甲基化通过抑制家蚕Bombyx mori中Bmara介导的几丁质酶表达来抑制几丁质降解并促进翅膀发育
DOI：
10.1186/s13072-020-00356-6
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发表时间：
2020-09
影响因子：
3.9
通讯作者：
Sichun Zheng
中科院分区：
文献类型：
--
作者：
Guanfeng Xu;Yangqin Yi;Hao Lyu;Chengcheng Gong;Qili Feng;Qisheng Song;Xuezhen Peng;Lin Liu;Sichun Zheng
BackgroundDNA methylation, as an essential epigenetic modification found in mammals and plants, has been implicated to play an important role in insect reproduction. However, the functional role and the regulatory mechanism of DNA methylation during insect organ or tissue development are far from being clear.ResultsHere, we found that DNA methylation inhibitor (5-aza-dC) treatment in newly molted pupae decreased the chitin content of pupal wing discs and adult wings and resulted in wing deformity ofBombyx mori. Transcriptome analysis revealed that the up-regulation of chitinase 10 (BmCHT10) gene might be related to the decrease of chitin content induced by 5-aza-dC treatment. Further, the luciferase activity assays demonstrated that DNA methylation suppressed the promoter activity ofBmCHT10by down-regulating the transcription factor, homeobox protein araucan (Bmara). Electrophoretic mobility shift assay, DNA pull-down and chromatin immunoprecipitation demonstrated that Bmara directly bound to theBmCHT10promoter. Therefore, DNA methylation is involved in keeping the structural integrity of the silkworm wings from unwanted chitin degradation, as a consequence, it promotes the wing development ofB. mori.ConclusionsThis study reveals that DNA methylation plays an important role in the wing development ofB. mori. Our results support that the indirect transcriptional repression of a chitin degradation-related geneBmCHT10by DNA methylation is necessary to keep the proper wing development inB. mori.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
P. Malik;A. Cashen
通讯作者：
P. Malik;A. Cashen
影响因子：
2.6
作者：
Glastad, K. M.;Hunt, B. G.;Goodisman, M. A. D.
通讯作者：
Goodisman, M. A. D.
DOI：
10.1016/j.pbiomolbio.2018.04.008
发表时间：
2018-04
影响因子：
3.8
作者：
H. Nijhout;Kenneth Z McKenna
通讯作者：
H. Nijhout;Kenneth Z McKenna
影响因子：
3.8
作者：
Guanfeng Xu;Jie Zhang;H. Lyu;Jia Liu;Yang Ding;Q. Feng;Q. Song;Sichun Zheng
通讯作者：
Guanfeng Xu;Jie Zhang;H. Lyu;Jia Liu;Yang Ding;Q. Feng;Q. Song;Sichun Zheng