Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism.
Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism.
复制标题
DOI：
10.1186/1471-2229-11-24
复制
发表时间：
2011-01-26
影响因子：
5.3
通讯作者：
Rosati C
中科院分区：
文献类型：
--
作者：
Brandi F;Bar E;Mourgues F;Horváth G;Turcsi E;Giuliano G;Liverani A;Tartarini S;Lewinsohn E;Rosati C
类胡萝卜素是植物代谢产物，不仅在光合作用中起重要作用，而且是决定花和果实色素沉着和香气的重要品质因子。为了研究桃（Prunus persica L. Batsch.）类胡萝卜素代谢与类异戊二烯及其他挥发性化合物的相关调控，以及类胡萝卜素双加氧酶在果肉颜色表型和挥发性成分差异中的作用，研究了果实发育过程中相关类胡萝卜素基因和代谢物的表达模式以及挥发性化合物含量的变化。研究了两个对照品种，黄肉质的‘红港’（RH）和它的白肉质突变体‘红港比安卡’（RHB）。两种基因型在中果皮组织中类胡萝卜素的积累有显著差异。RHB中类胡萝卜素含量较低，类去异戊二烯挥发物含量较高，这可能与类胡萝卜素裂解双加氧酶（CCD）活性的差异有关。事实上，相对于RH， ccd4转录水平在RHB的成熟后期显著更高。这两个基因型在一些类胡萝卜素和类异戊二烯转录物的表达模式上也表现出差异，这与这些转录物的反馈调控相一致。一种CCD酶抑制剂Abamine SG可以降低RHB果实中类异戊二烯和非类异戊二烯挥发物的水平，表明挥发物产生的复杂调控。ccd4的差异表达可能是桃果肉中类胡萝卜素和类胡萝卜素衍生挥发物积累的主要决定因素。更一般地说，双加氧酶似乎是控制桃果中挥发性成分的关键因素，因为经紫金素sg处理的“红港比安卡”果实中去异戊二烯类和其他挥发性类的含量大大降低。桃类胡萝卜素裂解酶在桃果实色素沉着和香气中的作用有待进一步研究。
Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism, as related to norisoprenoids and other volatile compounds in peach (Prunus persica L. Batsch.), and the role of carotenoid dioxygenases in determining differences in flesh color phenotype and volatile composition, the expression patterns of relevant carotenoid genes and metabolites were studied during fruit development along with volatile compound content. Two contrasted cultivars, the yellow-fleshed 'Redhaven' (RH) and its white-fleshed mutant 'Redhaven Bianca' (RHB) were examined. The two genotypes displayed marked differences in the accumulation of carotenoid pigments in mesocarp tissues. Lower carotenoid levels and higher levels of norisoprenoid volatiles were observed in RHB, which might be explained by differential activity of carotenoid cleavage dioxygenase (CCD) enzymes. In fact, the ccd4 transcript levels were dramatically higher at late ripening stages in RHB with respect to RH. The two genotypes also showed differences in the expression patterns of several carotenoid and isoprenoid transcripts, compatible with a feed-back regulation of these transcripts. Abamine SG - an inhibitor of CCD enzymes - decreased the levels of both isoprenoid and non-isoprenoid volatiles in RHB fruits, indicating a complex regulation of volatile production. Differential expression of ccd4 is likely to be the major determinant in the accumulation of carotenoids and carotenoid-derived volatiles in peach fruit flesh. More in general, dioxygenases appear to be key factors controlling volatile composition in peach fruit, since abamine SG-treated 'Redhaven Bianca' fruits had strongly reduced levels of norisoprenoids and other volatile classes. Comparative functional studies of peach carotenoid cleavage enzymes are required to fully elucidate their role in peach fruit pigmentation and aroma.
登录
查看更多内容
影响因子：
7.4
作者：
Diretto, Gianfranco;Al-Babili, Salim;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
4.4
作者：
Ahrazem, Oussama;Trapero, Almudena;Gomez-Gomez, Lourdes
通讯作者：
Gomez-Gomez, Lourdes
影响因子：
3
作者：
DERMEN, H;STEWART, RN
通讯作者：
STEWART, RN
影响因子：
5.3
作者：
Diretto, Gianfranco;Welsch, Ralf;Tavazza, Raffaela;Mourgues, Fabienne;Pizzichini, Daniele;Beyer, Peter;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
3.8
作者：
Ibdah, Mwafaq;Azulay, Yaniv;Lewinsohn, Efraim
通讯作者：
Lewinsohn, Efraim