NMR Studies of Metabolism of Cells and Perfused Organs
NMR Studies of Metabolism of Cells and Perfused Organs
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细胞和灌注器官代谢的核磁共振研究
DOI：
--
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. S. Cohen
中科院分区：
文献类型：
--
作者：
O. Kaplan;P. Cohen;J. S. Cohen
This chapter documents the application of NMR spectroscopic methods to studies of the metabolism of cells and perfused organs. Progress in this area is attested to by the sheer number of publications. This area of research stands alone, as a means of shedding light on the metabolism and pharmacology of cells and organs, but also exists as a basis for the applications of NMR spectroscopic methods to in vivo and clinical situations. Methods of obtaining cellular extracts are included, but the bulk of the chapter is devoted to the methods that have been developed to obtain spectra from intact cells perfused in the NMR tube. Such methods as embedding in gels, use of microcarrier beads, hollow fibers, and spheroids are described and compared. Most of this work involves 31P-NMR, but some 13C-NMR tracer studies are also reported. A section is also devoted to the consideration of proton NMR as a means to monitor metabolism, and the necessity of not only suppressing the water signal, but also of removing all signals of extracellular origin, which has recently been accomplished. Finally some work on perfused organs is described and collated into a table that could prove useful for the reader. The question arises as to the relevance of the results with perfused organs relative to those in vivo. As clinical spectroscopy develops, future work must address this question.
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