VSV-Based Vaccines Reduce Virus Shedding and Viral Load in Hamsters Infected with SARS-CoV-2 Variants of Concern.
VSV-Based Vaccines Reduce Virus Shedding and Viral Load in Hamsters Infected with SARS-CoV-2 Variants of Concern.
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DOI：
10.3390/vaccines10030435
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发表时间：
2022-03-12
期刊：
影响因子：
7.8
通讯作者：
Marzi A
中科院分区：
文献类型：
--
作者：
O'Donnell KL;Gourdine T;Fletcher P;Shifflett K;Furuyama W;Clancy CS;Marzi A
COVID-19大流行的持续发展可部分归因于SARS-CoV-2突变并引入新病毒变体的能力。其中一些变种有可能迅速传播并征服地球仪，称为关注变种（VOC）。在全球范围内实施的现有疫苗是基于祖先菌株，这导致随着这些VOC的出现，突破性感染的数量增加。必须用新开发的疫苗显示对VOC感染的保护。之前，我们评估了两种基于水泡性口炎病毒（VSV）的疫苗，它们单独表达SARS-CoV-2刺突蛋白（VSV-SARS 2）或与埃博拉病毒糖蛋白（VSV-SARS 2-EBOV）联合表达，并证明了它们的快速作用潜力。在这里，我们延长了攻击的时间;我们在感染前28天通过鼻内（IN）或肌肉内接种了三种SARS-CoV-2 VOC-α，β和δ变体。用VSV-SARS 2或VSV-SARS 2-EBOV的IN疫苗接种导致最高的保护效力，如通过疫苗接种的仓鼠的减少的病毒脱落和肺病毒载量所证明的。肺组织学分析显示，无论攻毒病毒如何，IN免疫动物的肺损伤最少。这些数据表明，基于VSV的疫苗不仅能够预防SARS-CoV-2 VOC引起的疾病，而且还能够减少病毒脱落。
The continued progression of the COVID-19 pandemic can partly be attributed to the ability of SARS-CoV-2 to mutate and introduce new viral variants. Some of these variants with the potential to spread quickly and conquer the globe are termed variants of concern (VOC). The existing vaccines implemented on a global scale are based on the ancestral strain, which has resulted in increased numbers of breakthrough infections as these VOC have emerged. It is imperative to show protection against VOC infection with newly developed vaccines. Previously, we evaluated two vesicular stomatitis virus (VSV)-based vaccines expressing the SARS-CoV-2 spike protein alone (VSV-SARS2) or in combination with the Ebola virus glycoprotein (VSV-SARS2-EBOV) and demonstrated their fast-acting potential. Here, we prolonged the time to challenge; we vaccinated hamsters intranasally (IN) or intramuscularly 28 days prior to infection with three SARS-CoV-2 VOC—the Alpha, Beta, and Delta variants. IN vaccination with either the VSV-SARS2 or VSV-SARS2-EBOV resulted in the highest protective efficacy as demonstrated by decreased virus shedding and lung viral load of vaccinated hamsters. Histopathologic analysis of the lungs revealed the least amount of lung damage in the IN-vaccinated animals regardless of the challenge virus. This data demonstrates the ability of a VSV-based vaccine to not only protect from disease caused by SARS-CoV-2 VOC but also reduce viral shedding.
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影响因子：
64.5
作者：
Hoffmann M;Krüger N;Schulz S;Cossmann A;Rocha C;Kempf A;Nehlmeier I;Graichen L;Moldenhauer AS;Winkler MS;Lier M;Dopfer-Jablonka A;Jäck HM;Behrens GMN;Pöhlmann S
通讯作者：
Pöhlmann S
影响因子：
7.8
作者：
Khong KW;Liu D;Leung KY;Lu L;Lam HY;Chen L;Chan PC;Lam HM;Xie X;Zhang R;Fan Y;To KK;Chen H;Yuen KY;Chan KH;Hung IF
通讯作者：
Hung IF
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
DOI：
10.1056/nejmoa2102214
发表时间：
2021-05-20
期刊：
The New England journal of medicine
影响因子：
--
作者：
Madhi SA;Baillie V;Cutland CL;Voysey M;Koen AL;Fairlie L;Padayachee SD;Dheda K;Barnabas SL;Bhorat QE;Briner C;Kwatra G;Ahmed K;Aley P;Bhikha S;Bhiman JN;Bhorat AE;du Plessis J;Esmail A;Groenewald M;Horne E;Hwa SH;Jose A;Lambe T;Laubscher M;Malahleha M;Masenya M;Masilela M;McKenzie S;Molapo K;Moultrie A;Oelofse S;Patel F;Pillay S;Rhead S;Rodel H;Rossouw L;Taoushanis C;Tegally H;Thombrayil A;van Eck S;Wibmer CK;Durham NM;Kelly EJ;Villafana TL;Gilbert S;Pollard AJ;de Oliveira T;Moore PL;Sigal A;Izu A;NGS-SA Group;Wits-VIDA COVID Group
通讯作者：
Wits-VIDA COVID Group
影响因子：
7.8
作者：
Loconsole D;Bisceglia L;Centrone F;Sallustio A;Accogli M;Dalfino L;Brienza N;Chironna M
通讯作者：
Chironna M