Stain-Free total-protein normalization enhances the reproducibility of Western blot data.
Stain-Free total-protein normalization enhances the reproducibility of Western blot data.
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DOI：
10.1016/j.ab.2022.114840
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发表时间：
2022-10-01
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
我们比较了三种常用的总蛋白归一化方法的准确性。与Ponceau S和Amido Black不同，Stain-Free方法在不同类型/品牌的western blotting膜和各种蛋白质负载上都是准确的。归一化到看家蛋白Actin和β-Tubulin可以匹配无染色方法的准确性。然而，与Actin或β-Tubulin相比，归一化到Stain-Free信号减少了可变性，从而提高了再现性，并将获得统计显著结果所需的样品数量减少了50%。无染色归一化可以提高重现性，从而提高Western Blot数据的可信度。
We compared the accuracy of three common methods of total protein normalization. The Stain-Free method was accurate across different types/brands of western blotting membrane and for various protein loads, unlike Ponceau S and Amido Black. Normalizing to the housekeeping proteins Actin and β-Tubulin could match the accuracy of the Stain-Free method. However, compared to Actin or β-Tubulin, normalizing to the Stain-Free signal reduced variability that led to enhanced reproducibility and a reduction in the number of samples needed to obtain statistically significant results by >50%. Stain-Free normalization can enhance the reproducibility and hence the confidence in Western Blot data.
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影响因子：
3.4
作者：
Ghosh R;Gilda JE;Gomes AV
通讯作者：
Gomes AV
影响因子：
2.9
作者：
Gilda JE;Gomes AV
通讯作者：
Gomes AV
影响因子：
2.9
作者：
Rivero-Gutierrez, B.;Anzola, A.;de Medina, F. Sanchez
通讯作者：
de Medina, F. Sanchez
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2.9
作者：
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通讯作者：
Ronn, Ola
影响因子：
2.9
作者：
Thacker, Jonathan S.;Yeung, Derrick H.;Mielke, John G.
通讯作者：
Mielke, John G.