Stain-Free total protein staining is a superior loading control to β-actin for Western blots.
Stain-Free total protein staining is a superior loading control to β-actin for Western blots.
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DOI：
10.1016/j.ab.2013.05.027
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发表时间：
2013-09-15
影响因子：
2.9
通讯作者：
Gomes AV
中科院分区：
文献类型：
--
作者：
Gilda JE;Gomes AV
使用蛋白质印迹的蛋白质的半定量通常涉及针对管家基因（如β-肌动蛋白）的标准化。最近，丽春红S和考马斯亮蓝染色都被证明是合适的替代管家基因作为加载控制。无染色总蛋白染色提供了无染色或脱色步骤的优点。使用无染色作为β-肌动蛋白或丽春红S蛋白染色剂的替代品的评价表明，无染色上级β-肌动蛋白，并且与丽春红S染色一样好或更好地作为蛋白质印迹的上样对照。
Semi-Quantification of proteins using Western blots typically involves normalization against housekeeping genes such as β-actin. More recently, ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain free staining as an alternative to β-actin or the protein stain ponceau S showed that Stain free staining was superior to β-actin and as good as or better than ponceau S staining as a loading control for Western blots.
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