Polypeptide hormone receptor phosphorylation: is there a role in receptor-mediated endocytosis of human growth hormone?
Polypeptide hormone receptor phosphorylation: is there a role in receptor-mediated endocytosis of human growth hormone?
复制标题
多肽激素受体磷酸化：在受体介导的人类生长激素内吞作用中是否有作用？
作者：
K. Asakawa;G. Grunberger;A. Mcelduff;P. Gorden
To determine whether receptor phosphorylation is a critical step in the internalization of polypeptide hormones and their receptors, we have studied a model system wherein insulin stimulates phosphorylation of its receptor and is also internalized. Using insulin as a positive control, we found that it stimulated a partially purified plasma membrane preparation of IM-9 lymphocytes to autophosphorylate its receptor and to catalyze the phosphorylation of a tyrosine-containing substrate. The human GH (hGH) receptor of the IM-9 lymphocytes, when coupled to [125I]iodo-hGH, migrated as a 140,000-dalton protein on polyacrylamide gel electrophoresis. This protein, in contrast to the insulin receptor, was not phosphorylated by the addition of hGH, nor did hGH stimulate this preparation to phosphorylate the tyrosine-containing substrate poly-(GluNa,Tyr)4:1, casein, or histone f2b under a variety of conditions. We conclude that receptor phosphorylation is not a critical intermediate in the receptor-mediated endocytosis of hGH and probably other polypeptide hormones and growth factors.
佛波酯诱导的过度磷酸化与 HL60 细胞中转铁蛋白膜受体可逆调节的关联。
DOI：
10.1073/pnas.81.7.2016
发表时间：
1984
影响因子：
11.1
作者：
May,WS;Jacobs,S;Cuatrecasas,P
通讯作者：
Cuatrecasas,P
人血小板衍生生长因子刺激瑞士小鼠 3T3 细胞中的蛋白质磷酸化。
DOI：
10.1016/0006-291x(81)91700-9
发表时间：
1981
影响因子：
3.1
作者：
Nishimura,J;Deuel,TF
通讯作者：
Deuel,TF