A taxonomic study of Quercus langbianensis complex based on morphology and DNA barcodes of classic and next generation sequences.

A taxonomic study of Quercus langbianensis complex based on morphology and DNA barcodes of classic and next generation sequences.
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DOI:
10.3897/phytokeys.95.21126
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发表时间:
2018
期刊:
影响因子:
1.4
通讯作者:
Yahara T
Yahara T
中科院分区:
生物学3区
文献类型:
--
作者:
Binh HT;Ngoc NV;Tagane S;Toyama H;Mase K;Mitsuyuki C;Strijk JS;Suyama Y;Yahara T

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根据野外观察、植物标本形态学比较和分子生物学分析,对越南和柬埔寨的兰边栎(Quercus langbianensis)及其近缘种进行了分类学修订。基于rbcL、matK和ITS区的贝叶斯推断和利用多个简单重复序列间(inter-simple sequence repeat,ISSR)引物扩增的全基因组序列的邻接树(Neighbour-joining tree,MIG-seq),作者在越南和柬埔寨的复合体中识别出10个种,其中3个是本文新描述的:Q. baolamensissp. nov.,Q. bidoupensissp. nov.和Q. honbaensissp. nov.这些新种的表型与Q.浪边湾具披针形至倒披针形叶,叶缘4-5/6锯齿状,叶尖锐尖或渐尖,壳斗苞片5-9轮排列,但形态和发育上均不同。在分子系统发育重建中,Q. bidoupensis与任何其他物种都不接近。在贝叶斯树中,Q. honbaensis是Q. blaoensis和Q. Camusiae,这3个种与Q.浪边湾字符串,而Baolamensis与Q.浪边湾在贝叶斯树和MIG-seq树中。此外,Q. cambodiensis和Q. baniensis以前减少到Q.浪边湾lat.被认为是不同的物种。有6个物种需要进行选型,在此进行。
The taxonomy of Quercus langbianensis and its relatives in Vietnam and Cambodia have been revised based on evidence obtained from field observations, morphological comparison of herbarium specimens and molecular analyses using both classic and next generation DNA markers. Based on Bayesian inference using rbcL, matK and ITS regions and Neighbour-joining tree using genome-wide sequences amplified with multiplexed inter-simple sequence repeat (ISSR) primers (MIG-seq), the authors recognised ten species in the complex in Vietnam and Cambodia, three of which are newly described in this paper: Q. baolamensis sp. nov., Q. bidoupensis sp. nov. and Q. honbaensis sp. nov. These new species are all phenotypically similar to Q. langbianensis s. str. in having lanceolate to oblanceolate leaf shape, upper 4–5/6–serrated leaf margin, acute or acuminate leaf apex and bracts of cupule arranged in 5–9 rings but distinguished both morphologically and phylogenetically. In molecular phylogenetic reconstructions, Q. bidoupensis is not close to any other species. In the Bayesian tree, Q. honbaensis is sister to both Q. blaoensis and Q. camusiae that are found in the same locality but morphologically distinct and those three species are sister to Q. langbianensis s. str., while Quercus baolamensis is not sister to Q. langbianensis s. str. in both the Bayesian tree and MIG-seq tree. In addition, Q. cambodiensis and Q. baniensis previously reduced to Q. langbianensis s. lat. have been recognised as distinct species. Six species were in need of lectotypification and that is undertaken herein.
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