Suppression of reflex urethral responses by sacral dermatome stimulation in an acute spinalized feline model.
Suppression of reflex urethral responses by sacral dermatome stimulation in an acute spinalized feline model.
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DOI：
10.1002/nau.20717
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发表时间：
2010-03
影响因子：
2
通讯作者：
Gustafson, Kenneth J.
中科院分区：
文献类型：
--
作者：
Mariano, Timothy Y.;Bhadra, Narendra;Gustafson, Kenneth J.
关键词：
外尿道括约肌（EUS）反射性收缩是神经损伤或疾病后排尿功能障碍的主要组成部分。异常的尿道反射可以阻止排尿并引起严重的医学并发症。在泌尿生殖系统研究中描述这些尿道反射对于评估新的药理学或神经修复方法是必要的。本研究的目的是在急性脊髓猫科动物中产生尿道反射，量化这些反射，并通过骶骨皮节的电刺激来抑制它们。这项研究包括8只公猫。用-氯氯蔗糖或戊巴比妥钠维持麻醉。脊髓在T10和T12之间横切，神经袖电极置于硬膜外骶S2根，以提供膀胱激活。记录膀胱收缩时和收缩后的膀胱和尿道压力。电刺激应用于非侵入性的骶骨皮体与商业表面电极。六只猫的尿道反射被一致地激发。量化相应的尿道压力峰值。尿道反射活动的假定指标，如反射压力峰值的速率和平均幅度，与标准尿动力学变量显著相关。电刺激三只猫的骶皮节抑制尿道反射。这些发现在急性脊髓猫准备证明了一种非侵入性手段抑制不希望的尿道反射。将这项工作转化为临床应用，可以改进神经假体以恢复膀胱功能，并加强对人类异常尿道反射的治疗。
Reflex contractions of the external urethral sphincter (EUS) are a major component of voiding dysfunction after neurological injury or disease. Aberrant urethral reflexes can prevent voiding and cause serious medical complications. Characterizing these urethral reflexes during genitourinary studies is necessary for evaluating novel pharmacological or neuroprosthetic approaches. The objectives of the present study were to generate urethral reflexes in the acute spinal feline, to quantify these reflexes, and to suppress them with electrical stimulation of the sacral dermatomes. This study comprised eight male cats. Anaesthesia was maintained with alpha-chloralose or sodium pentobarbital. The spinal cord was transected between T10 and T12, and nerve cuff electrodes were placed on the extradural S2 sacral roots to provide bladder activation. Bladder and urethral pressures were recorded during and after bladder contractions. Electrical stimulation was applied non-invasively to the sacral dermatomes with commercial surface electrodes. Urethral reflexes were elicited consistently in six cats. The corresponding urethral pressure spikes were quantified. Putative metrics of urethral reflex activity such as the rate and average magnitude of reflex pressure spikes correlated significantly with standard urodynamic variables. Electrical stimulation of the sacral dermatomes suppressed urethral reflexes in three cats. These findings in an acute spinal feline preparation demonstrate a non-invasive means of suppressing undesirable urethral reflexes. Translation of this work to clinical use could improve neuroprostheses for restoring bladder function and enhance treatment of aberrant urethral reflexes in humans.
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DOI：
10.1152/ajprenal.00443.2007
发表时间：
2008-03-01
影响因子：
4.2
作者：
Tai, Changfeng;Shen, Bing;de Groat, William C.
通讯作者：
de Groat, William C.
影响因子：
2
作者：
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影响因子：
2.7
作者：
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Emmanuel, Anton V.
影响因子：
2
作者：
Boger, Adam;Bhadra, Narendra;Gustafson, Kenneth J.
通讯作者：
Gustafson, Kenneth J.
影响因子：
2.4
作者：
Bajd, T;Munih, M;Cikajlo, I
通讯作者：
Cikajlo, I