Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies inTrypanosoma cruzi-infected mice
Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies inTrypanosoma cruzi-infected mice
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克氏锥虫感染小鼠的循环转唾液酸酶活性和转唾液酸酶抑制抗体
DOI：
10.1007/bf00932022
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发表时间：
2004
影响因子：
2
通讯作者：
L. Pontes
中科院分区：
文献类型：
--
作者：
N. Alcântara;L. Pontes
在克氏锥虫感染小鼠的血清和血液中证实了寄生虫衍生的转唾液酸酶（TS）活性。血清TS活性水平与BALB/c和瑞士小鼠在感染的初始阶段的寄生虫血症。然而，在后期阶段的TS活动水平下降，尽管增加寄生虫血症。这与循环TS抗体的出现相吻合。另一方面，TS活性与无胸腺裸小鼠寄生虫血症之间始终存在良好的相关性。高寄生虫血症和低TS活性小鼠血清抑制TS活性。纯化血清IgG也观察到了这种抑制作用，并且它被葡萄球菌蛋白A吸收，表明它是由抗TS IgG抗体引起的。这些抗体抑制不溶性TS的酶活性，表明它们通过干扰催化位点而不是通过聚集酶来起作用。然而，抑制性抗体的存在并不能阻止BALB/c小鼠寄生虫血症的进展。
Parasite-derived trans-sialidase (TS) activity was demonstrated in the serum and blood ofTrypanosoma cruzi-infected mice. Serum TS activity levels correlated well with parasitemia in BALB/c and Swiss mice during the initial stages of the infection. However, in later stages the TS activity levels decreased despite increasing parasitemia. This coincided with the appearance of circulating TS antibodies. On the other hand, there was always a good correlation between TS activity and parasitemia in athymic nude mice. Sera from mice with high parasitemia and low TS activity inhibited TS activity in vitro. The inhibition was also observed with purified serum IgG, and it was absorbed by staphylococcal protein A, indicating that it was caused by anti-TS IgG antibodies. These antibodies inhibited the enzymatic activity of insolubilized TS, indicating that they act by interfering with the catalytic site rather than by aggregating the enzyme. The presence of inhibitory antibodies, however, did not prevent the progression of parasitemia in BALB/c mice.
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宿主细胞上的唾液酸和锥虫上的转唾液酸酶介导克氏锥虫入侵。
影响因子：
1.5
作者：
Ming,M;Chuenkova,M;Ortega-Barria,E;Pereira,ME
通讯作者：
Pereira,ME
克氏锥虫中发育调节的神经氨酸酶活性。
DOI：
10.1126/science.6338592
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pereira,ME
通讯作者：
Pereira,ME
锥虫唾液酸酶/反式唾液酸酶活性和利什曼原虫金属蛋白酶基因同源物在内锥虫属中的联合出现。
影响因子：
1.5
作者：
Medina-Acosta,E;Paul,S;Tomlinson,S;Pontes-de-Carvalho,LC
通讯作者：
Pontes-de-Carvalho,LC
影响因子：
15.9
作者：
LIBBY, P;ALROY, J;PEREIRA, MEA
通讯作者：
PEREIRA, MEA