Two major antigenic polypeptides of molluscum contagiosum virus.
Two major antigenic polypeptides of molluscum contagiosum virus.
复制标题
传染性软疣病毒的两种主要抗原多肽。
DOI：
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发表时间：
1998
影响因子：
6.4
通讯作者：
Y. Ueda
中科院分区：
文献类型：
--
作者：
T. Watanabe;S. Morikawa;K. Suzuki;T. Miyamura;K. Tamaki;Y. Ueda
用12例牛痘患者血清对传染性软疣病毒（MCV）转入牛痘载体表达系统进行筛选。免疫印迹法检测到两个70和34 kDa的重组蛋白，分别定位于开放阅读框MC133L和MC084L。在70 kda的MCV蛋白的c端与牛痘和天花病毒的14 kda融合蛋白之间，以及34 kda的MCV蛋白与37.5 kda的牛痘和天花病毒的病毒膜相关蛋白之间，发现了一致的位点。制备了兔抗这两种蛋白的血清。免疫荧光研究表明，70-和34-kDa蛋白主要在重组病毒感染的HeLa细胞表面表达，表明有可能插入到膜中。在免疫电镜下，抗70-kDa蛋白的抗血清对MCV膜有明显的标记作用，而抗34-kDa蛋白的抗血清对MCV膜没有明显的标记作用。
A library of molluscum contagiosum virus (MCV) transferred into the cowpox vector expression system was screened with 12 sera from molluscum patients. Two recombinant proteins of 70 and 34 kDa were detected by immunoblotting and mapped to the open-reading frames MC133L and MC084L, respectively. Consensus sites were found between the C-terminus of the 70-kDa MCV protein and the 14-kDa fusion protein of vaccinia and variola virus, and between the 34-kDa MCV protein and the 37.5-kDa viral membrane-associated protein of vaccinia and variola virus. Rabbit antisera against these two proteins were prepared. An immunofluorescence study demonstrated that the 70- and 34-kDa proteins were predominantly expressed on the surface of recombinant virus-infected HeLa cells, indicating the potential to be inserted into the membrane. On immunoelectron microscopy, antiserum against 70-kDa protein showed significant labeling of the MCV membrane, while the antiserum against 34-kDa protein failed to do so.
传染性软疣病毒的复制。
影响因子：
3.7
作者：
Buller,RM;Burnett,J;Chen,W;Kreider,J
通讯作者：
Kreider,J