A new era of the vaginal microbiome: advances using next-generation sequencing.
A new era of the vaginal microbiome: advances using next-generation sequencing.
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阴道微生物组的新时代：新一代测序技术的进步。
DOI：
10.1002/cbdv.201100359
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发表时间：
2012-05
影响因子：
2.9
通讯作者：
Buck, Gregory A.
中科院分区：
文献类型：
--
作者：
Fettweis, Jennifer M.;Serrano, Myrna G.;Girerd, Philippe H.;Jefferson, Kimberly K.;Buck, Gregory A.
关键词：
直到最近，栖息在人类阴道的细菌种类主要是用以生物为中心的方法来研究的。了解这些细菌物种如何相互作用以及宿主阴道上皮对于更全面地了解阴道健康至关重要。分子方法已经导致鉴定与细菌性阴道病相关的未培养细菌分类群。在这里，我们回顾了阴道微生物组的最新研究，并讨论了诸如下一代测序的应用等与培养无关的方法如何推动该领域的发展，并改变了我们对阴道健康如何定义的理解。这项工作可能会改善患有或有可能患有阴道失衡、妊娠并发症和性获得性感染的妇女的诊断工具和治疗方法。这些方法也可能改变我们对宿主遗传因素、生理状况（如更年期）和环境暴露（如吸烟、抗生素使用）如何影响阴道微生物群的理解。
Until recently, bacterial species that inhabit the human vagina have been primarily studied using organism-centric approaches. Understanding how these bacterial species interact with each other and the host vaginal epithelium is essential for a more complete understanding of vaginal health. Molecular approaches have already led to the identification of uncultivated bacterial taxa associated with bacterial vaginosis. Here, we review recent studies of the vaginal microbiome and discuss how culture-independent approaches, such as applications of next-generation sequencing, are advancing the field and shifting our understanding of how vaginal health is defined. This work may to lead to improved diagnostic tools and treatments for women who suffer from, or are at risk for, vaginal imbalances, pregnancy complications, and sexually acquired infections. These approaches may also transform our understanding of how host genetic factors, physiological conditions (e.g. menopause) and environmental exposures (e.g. smoking, antibiotic usage) influence the vaginal microbiome.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
4.2
作者：
De Backer, Ellen;Verhelst, Rita;Vaneechoutte, Mario
通讯作者：
Vaneechoutte, Mario
影响因子：
--
作者：
BOURNE, A
通讯作者：
BOURNE, A
影响因子：
2.3
作者：
Fredricks, David N.
通讯作者：
Fredricks, David N.
影响因子：
9.4
作者：
Fredricks, David N.;Fiedler, Tina L.;Marrazzo, Jeanne M.
通讯作者：
Marrazzo, Jeanne M.