Role of Plasmodium berghei ookinete surface and oocyst capsule protein, a novel oocyst capsule-associated protein, in ookinete motility.
Role of Plasmodium berghei ookinete surface and oocyst capsule protein, a novel oocyst capsule-associated protein, in ookinete motility.
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DOI：
10.1186/s13071-021-04868-2
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发表时间：
2021-07-21
影响因子：
3.2
通讯作者：
Ikadai H
中科院分区：
文献类型：
--
作者：
Nakayama K;Kimura Y;Kitahara Y;Soga A;Haraguchi A;Hakozaki J;Sugiyama M;Kusakisako K;Fukumoto S;Ikadai H
疟原虫属，在传播之前，必须首先在蚊子身上发展。在摄入感染的血液后，配子在蚊子腔中形成，随后受精并将所得的合子分化成能动的动合子。在24小时内的血液摄入，这些动卵细胞穿越蚊子上皮细胞和下中肠基膜，在那里他们分化成无柄卵囊的保护胶囊。我们确定了一个动合子表面和卵囊膜蛋白（OSCP），它参与动合子运动以及卵囊膜的形成。我们发现，敲除寄生虫中的OSCP降低了动合子的滑行运动，并逐渐减少卵囊的数量。在摄食血液后第15天，卵囊壁明显变薄。此外，添加抗OSCP抗体降低了野生型动合子在体外的滑行速度。将抗OSCP抗体添加到受感染的血粉中也导致卵囊形成减少。这些发现可能有助于开发疟疾传播阻断工具。
Plasmodium sp., which causes malaria, must first develop in mosquitoes before being transmitted. Upon ingesting infected blood, gametes form in the mosquito lumen, followed by fertilization and differentiation of the resulting zygotes into motile ookinetes. Within 24 h of blood ingestion, these ookinetes traverse mosquito epithelial cells and lodge below the midgut basal lamina, where they differentiate into sessile oocysts that are protected by a capsule. We identified an ookinete surface and oocyst capsule protein (OSCP) that is involved in ookinete motility as well as oocyst capsule formation. We found that knockout of OSCP in parasite decreases ookinete gliding motility and gradually reduces the number of oocysts. On day 15 after blood ingestion, the oocyst wall was significantly thinner. Moreover, adding anti-OSCP antibodies decreased the gliding speed of wild-type ookinetes in vitro. Adding anti-OSCP antibodies to an infected blood meal also resulted in decreased oocyst formation. These findings may be useful for the development of a transmission-blocking tool for malaria.
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影响因子：
3.1
作者：
Ukegbu CV;Akinosoglou KA;Christophides GK;Vlachou D
通讯作者：
Vlachou D
影响因子：
3.8
作者：
Kumar, S;Barillas-Mury, C
通讯作者：
Barillas-Mury, C
影响因子：
4.8
作者：
Trisnadi, Nathanie;Barillas-Mury, Carolina
通讯作者：
Barillas-Mury, Carolina
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影响因子：
3.2
作者：
Sasaki H;Sekiguchi H;Sugiyama M;Ikadai H
通讯作者：
Ikadai H
影响因子：
2.8
作者：
Smith RC;Vega-Rodríguez J;Jacobs-Lorena M
通讯作者：
Jacobs-Lorena M