Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment.
Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment.
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泰国红树林沉积物中耐酸真菌的生物活性代谢产物。
DOI：
10.3389/fmicb.2020.609952
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Gao H;Wang Y;Luo Q;Yang L;He X;Wu J;Kachanuban K;Wilaipun P;Zhu W;Wang Y
耐酸真菌是一种潜在的极端微生物资源，但其活性代谢产物的研究报道较少。从泰国红树林沉积物中分离出嗜酸/耐酸真菌（n = 237）。利用真菌鉴定技术（包括形态观察、化学筛选和序列比较）对所有菌株进行鉴定，并选择41个代表菌株进行系统发育关系分析（ITS rDNA、β-微管蛋白、钙调蛋白和肌动蛋白基因序列）。在子囊菌门的四个分类目中鉴定出七个属--青霉属、曲霉属、篮状菌属、枝孢属、异点霉属、链格孢属和木霉属，青霉属、曲霉属和篮状菌属是优势属。耐酸性进行了评估，95%的菌株可以生长在极端酸性条件下（pH 2）。6株不能在pH 7下生存的嗜酸性真菌，亲缘关系非常近，均属于篮状菌属。这是首次报道该属植物的嗜酸特性。对发酵液的抑菌、抗肿瘤、抗病毒活性进行了评价。近四分之三的浸提液显示出细胞毒性活性，而不到四分之一的浸提液显示出抗菌或抗H1N1活性。典型的酸性真菌草酸青霉OUCMDZ-5207在pH 3和pH 7条件下表现出相似的生长，但其化学多样性完全不同。仅在pH 3时获得的OUCMDZ-5207代谢产物被鉴定为四氢金蓝素（1）、黄蓝素（2）和金蓝素（3），其中金蓝素对A549细胞显示出较强的选择性抑制作用，IC 50值为5.67 μM。这些结果表明，酸胁迫可以激活沉默基因簇，扩大次生代谢产物的多样性，泰国红树林沉积物中耐酸/嗜酸微生物资源的生物勘探可能会导致发现具有潜在药用价值的化合物。
Despite being potentially useful extremophile resources, there have been few reports on acid-tolerant fungi and their bioactive metabolites. Acidophilic/aciduric fungi (n = 237) were isolated from Thai mangrove sediments in an acidic medium. Using fungal identification technology (including morphologic observation, chemical screening, and sequence comparisons) all the isolates were identified and 41 representative isolates were selected for analysis of the phylogenetic relationships (ITS rDNA, β-tubulin, calmodulin, and actin gene sequences). There were seven genera identified – Penicillium; Aspergillus; Talaromyces; Cladosporium; Allophoma; Alternaria; and Trichoderma – in four taxonomic orders of the phylum Ascomycota, and Penicillium, Aspergillus, and Talaromyces were the dominant genera. Acidity tolerance was evaluated and 95% of the isolates could grow under extremely acidic conditions (pH 2). Six strains were classed as acidophilic fungi that cannot survive under pH 7, all of which had an extraordinarily close genetic relationship and belonged to the genus Talaromyces. This is the first report on the acidophilic characteristics of this genus. The antimicrobial, anti-tumor, and antiviral activities of the fermentation extracts were evaluated. Nearly three-quarters of the extracts showed cytotoxic activity, while less than a quarter showed antimicrobial or anti-H1N1 activity. The typical aciduric fungus Penicillium oxalicum OUCMDZ-5207 showed similar growth but completely different chemical diversity at pH 3 and 7. The metabolites of OUCMDZ-5207 that were obtained only at pH 3 were identified as tetrahydroauroglaucin (1), flavoglaucin (2), and auroglaucin (3), among which auroglaucin showed strong selective inhibition of A549 cells with an IC50 value of 5.67 μM. These results suggest that acid stress can activate silent gene clusters to expand the diversity of secondary metabolites, and the bioprospecting of aciduric/acidophilic microorganism resources in Thai mangrove sediments may lead to the discovery of compounds with potential medicinal applications.
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影响因子：
5.1
作者：
Gao J;León F;Radwan MM;Dale OR;Husni AS;Manly SP;Lupien S;Wang X;Hill RA;Dugan FM;Cutler HG;Cutler SJ
通讯作者：
Cutler SJ
DOI：
10.1099/ijs.0.022038-0
发表时间：
2011-02-01
影响因子：
2.8
作者：
Am-In, Somjit;Limtong, Savitree;Jindamorakot, Sasitorn
通讯作者：
Jindamorakot, Sasitorn
影响因子：
4.4
作者：
GLASS, NL;DONALDSON, GC
通讯作者：
DONALDSON, GC
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
影响因子：
5.6
作者：
Kim KS;Cui X;Lee DS;Ko W;Sohn JH;Yim JH;An RB;Kim YC;Oh H
通讯作者：
Oh H