Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.
Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.
复制标题
红密孔菌胞内蛋白提取物辅助纳米金生物合成及其催化4-硝基苯胺降解
DOI：
10.1186/s11671-015-0856-9
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发表时间：
2015
影响因子：
--
通讯作者：
Wu P
中科院分区：
文献类型：
--
作者：
Shi C;Zhu N;Cao Y;Wu P
绿色合成金纳米粒子是纳米技术领域的研究热点。生物合成路线被认为是环境友好和具有成本效益的。本研究探讨了利用血红密孔菌胞内蛋白提取物（IPE）合成金纳米粒子的可行性。利用紫外可见光谱、X射线衍射（XRD）和透射电子显微镜（TEM）分别对生成粒子的形成、结晶性质、形貌和尺寸进行了分析。紫外-可见光谱在520 - 560 nm处显示出强吸收峰，表明形成了AuNPs。XRD分析显示，所形成的AuNP在性质上是纯结晶的。TEM观察表明，在不同条件下合成的金纳米粒子具有不同的形状，包括球形、假球形、三角形、截顶三角形、五边形和六边形，从几纳米到几百纳米不等。随着IPE添加量从10 mL增加到80 mL，AuNPs的平均尺寸从61.47 nm减小到29.30 nm。当初始金离子浓度从0.5 mM变化到2.0 mM时，平均尺寸从25.88 nm上升到51.99 nm。与溶液pH的情况一样，溶液pH为2.0时，平均尺寸为84.29 nm，溶液pH为12.0时，平均尺寸减小至6.07 nm。傅里叶变换红外光谱（FTIR）分析表明，纳米金颗粒表面的羟基、胺基和羧基等官能团参与了金离子的还原和纳米金颗粒的稳定化。结果表明，平均粒径为6.07 nm的0.019 mg AuNPs可在6 min内催化12.5 μmol的对硝基苯胺完全降解，且随着AuNPs的加入，降解速率显著提高。结果表明，血红拟青霉的IPE可用于金纳米粒子的环境友好合成。本文的在线版本（doi：10.1186/s11671-015-0856-9）包含补充材料，可供授权用户使用。
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs’ synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs. The online version of this article (doi:10.1186/s11671-015-0856-9) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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DOI：
10.1016/j.colsurfa.2012.08.026
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