Inhibition of central neurotransmitter release by ω-conotoxin GVIA, a peptide modulator of the N-type voltage-sensitive calcium channel
Inhibition of central neurotransmitter release by ω-conotoxin GVIA, a peptide modulator of the N-type voltage-sensitive calcium channel
复制标题
ω-芋螺毒素 GVIA（N 型电压敏感钙通道的肽调节剂）抑制中枢神经递质释放
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Hertting
中科院分区：
文献类型：
--
作者：
D. Dooley;A. Lupp;G. Hertting
总结氚溢出，由电刺激诱发[3 H]多巴胺-、[3 H]-去甲肾上腺素-、[3 H]-5-羟色胺-和[3 H]-乙酰胆碱-标记的切片从兔中枢神经系统的不连续区域制备，共芋螺毒素GVIA抑制（2 min; 2 ms，3 Hz，5 V/cm和24 mA）39- 50（ω-CT; 5 nmol/l），一种N型电压敏感性钙通道（N-VSCC）的肽调节剂。使用ω-CT（5 nmol/l）和[3 H]-去甲肾上腺素标记的海马切片的其他实验表明，ω-CT诱导的抑制具有时间依赖性，缓冲液钙浓度与ω-CT之间存在竞争性拮抗作用，长时间电刺激（15 min; 0.4 Hz）对ω-CT诱导的抑制无影响。这些不同的结果表明：1）ω-CT与钙竞争N-VSCC，2）ω-CT的抑制作用与N-VSCC的门控状态无关，3）N-VSCC的分子性质在中枢神经递质系统中可能相似或相同。ω-CT在研究N-VSCC参与神经递质释放方面似乎是一种有用的药理学工具。
SummaryTritium overflows, evoked by electrical stimulation (2 min; 2 ms, 3 Hz, 5 V/cm, and 24 mA) of [3H]dopamine-, [3H]-noradrenaline-, [3H]-5-hydroxytrypt-amine, and [3H]-acetylcholine-labeled slices prepared from discrete regions of the rabbit central nervous system, were inhibited 39–50% by co-conotoxin GVIA (ω-CT; 5 nmol/l), a peptide modulator of the N-type voltage-sensitive calcium channel (N-VSCC). Additional experiments using ω-CT (5 nmol/l) and [3H]-noradrenaline-labeled hippocampal slices indicated the time dependence of ω-CT-induced inhibition, the competitive antagonism between buffer calcium concentration and ω-CT, and the lack of effect of prolonged electrical stimulation (15 min; 0.4 Hz) on ω-CT-induced inhibition. These various results suggest that 1) ω-CT competes with calcium for the N-VSCC, 2) the inhibitory effects of ω-CT are independent of the gating state of the N-VSCC, and 3) the molecular nature of the N-VSCC may be similar or identical across central neurotransmitter systems. ω-CT appears to be a useful pharmacological tool in studying the involvement of the N-VSCC in neurotransmitter release.
二氢吡啶结合位点是电压敏感的钙通道吗？
影响因子：
6.1
作者：
Miller,RJ;Freedman,SB
通讯作者：
Freedman,SB
影响因子：
56.9
作者：
MILLER, RJ
通讯作者：
MILLER, RJ
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rivier,J;Galyean,R;Gray,WR;Azimi-Zonooz,A;McIntosh,JM;Cruz,LJ;Olivera,BM
通讯作者：
Olivera,BM