Effects of sex hormone rescue on gametogenesis in allotriploid crucian carp
Effects of sex hormone rescue on gametogenesis in allotriploid crucian carp
复制标题
性激素拯救对异源三倍体鲫鱼配子发生的影响
DOI：
10.1016/j.aquaculture.2022.738645
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发表时间：
2022-07
期刊：
影响因子：
4.5
通讯作者：
Shaojun Liu
中科院分区：
文献类型：
--
作者：
Jing Wang;Wen Wang;Jihong Li;Ziye Luo;Zhanxin Li;Mingli Cha;Caixia Xiang;Kaikun Luo;Rurong Zhao;Shaojun Liu
性激素在配子发生中起重要作用。在繁殖季节，不育异源三倍体鲫（3 nAT）的性激素水平显著低于可育二倍体红鲫（2nRCC）。采用性激素拯救法研究性激素与3 nAT不育的关系。在繁殖季节之前的三个月，3 nAT雌性和雄性分别喂食性激素雌二醇和睾酮。在繁殖季节，被拯救的3 nAT鱼（re-3 nAT）表现出正常的生殖行为，雌性re-3 nAT和雄性re-3 nAT的雌二醇和睾酮水平与二倍体对照（2nRCC）相似。vtg基因，这是与肝脏合成的卵黄蛋白原在女性，和genesspef 2，tekt 1，andift 27，这是与精子鞭毛的形成，在re-3 nAT相比，3 nAT显着上调。在人工辅助生殖下，re-3 nAT个体产生了一些不同大小的卵子和结构异常的精子。然而，很少有个体具有授精能力。无论是自交re-3 nAT产生的受精卵，也没有通过与2nRCC产生的单倍体配子杂交re-3 nAT产生的受精卵表现出正常的胚胎发育。因此，尽管性激素处理导致re-3 nAT产生异常配子，但没有发育出有活力的后代，这意味着re-3 nAT保持不育。这些结果表明，性激素影响配子成熟，但不是三倍体不育的决定因素。
Sex hormones play an important role in gametogenesis. During the breeding season, sex hormone levels in the sterile allotriploid crucian carp (3nAT) were significantly lower than those in the fertile diploid red crucian carp (2nRCC). Sex hormone rescue was performed to study the relationship between sex hormones and 3nAT sterility. For three months before breeding season, 3nAT females and males were fed the sex hormones estradiol and testosterone, respectively. During breeding season, hormone-rescued 3nAT fish (re-3nAT) showed normal reproductive behavior, and levels of estradiol in female re-3nAT and testosterone in male re-3nAT were similar to those of the diploid control (2nRCC). The vtg gene, which is associated with the hepatic synthesis of vitellogenin in the female, and the genesspef2, tekt1,andift27, which are associated with the formation of the sperm flagellum, were significantly upregulated in re-3nAT as compared to 3nAT. Under artificially assisted reproduction, re-3nAT individuals produced a few eggs of different sizes and sperm with abnormal structures. However, few individuals had insemination ability. Neither zygotes produced by self-crossing re-3nAT nor those produced by crossing re-3nAT with the haploid gametes produced by 2nRCC exhibited normal embryonic development. Thus, although sex hormone treatment led to the production of abnormal gametes by re-3nAT, viable offspring did not develop, meaning that re-3nAT remained sterile. These results indicated that sex hormones affected gamete maturation but were not the determinant of triploid sterility.
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DOI：
10.3109/10409238209108706
发表时间：
1982-03
期刊：
CRC critical reviews in biochemistry
影响因子：
--
作者：
D. Shapiro
通讯作者：
D. Shapiro
基因编辑揭示性类固醇在鱼类性别决定和分化中的作用
影响因子：
2.7
作者：
Zhou Linyan;Li Minghui;Wang Deshou
通讯作者：
Wang Deshou
影响因子：
6.2
作者：
C. Tyler;J. Sumpter
通讯作者：
C. Tyler;J. Sumpter
DOI：
10.1016/s1546-5098(08)60202-2
发表时间：
1988
期刊：
Fish Physiology
影响因子：
--
作者：
T. Mommsen;P. Walsh
通讯作者：
T. Mommsen;P. Walsh
影响因子：
2.8
作者：
Lai, Xiao-Jian;Li, Zhong-Qin;Wang, Yi-Lei
通讯作者：
Wang, Yi-Lei