The nontoxic natural compound Curcumin exerts anti-proliferative, anti-migratory, and anti-invasive properties against malignant gliomas.
The nontoxic natural compound Curcumin exerts anti-proliferative, anti-migratory, and anti-invasive properties against malignant gliomas.
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DOI：
10.1186/1471-2407-10-491
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发表时间：
2010-09-14
期刊：
影响因子：
3.8
通讯作者：
Weissenberger J
中科院分区：
文献类型：
--
作者：
Senft C;Polacin M;Priester M;Seifert V;Kögel D;Weissenberger J
人们不断寻求新的药物来提高恶性胶质瘤患者的生存率。理想的物质将选择性地靶向肿瘤细胞，而不会引起毒副作用。在这里，我们报告的抗增殖，抗迁移，抗侵袭特性的天然，无毒的化合物姜黄素在五个人胶质母细胞瘤（GBM）细胞系在体外观察。我们使用单层伤口愈合试验、改良的Boyden室trans-well试验和细胞生长试验来量化在不存在或存在不同浓度的姜黄素的情况下的细胞迁移、侵袭和增殖。转录因子磷酸-STAT 3的水平，姜黄素的一个潜在的目标，通过ELISA测定。通过实时定量PCR分析随后对调节细胞周期的基因转录的影响。通过半胱天冬酶测定确定对细胞凋亡的影响。姜黄素有效地抑制GBM细胞增殖以及在所有细胞系中的迁移和侵袭，这取决于剂量。同时，生物活性磷酸化STAT 3的水平降低，并与细胞周期调节基因c-Myc和增殖标记Ki-67的转录减少相关，这表明姜黄素减缓肿瘤生长的潜在机制。姜黄素是数百万人每天饮食的一部分，没有已知的毒副作用。我们的数据显示，姜黄素在体外对GBM细胞具有抗增殖、抗迁移和抗侵袭特性。这些结果保证了进一步的体内分析，并表明姜黄素在治疗恶性胶质瘤中的潜在作用。
New drugs are constantly sought after to improve the survival of patients with malignant gliomas. The ideal substance would selectively target tumor cells without eliciting toxic side effects. Here, we report on the anti-proliferative, anti-migratory, and anti-invasive properties of the natural, nontoxic compound Curcumin observed in five human glioblastoma (GBM) cell lines in vitro. We used monolayer wound healing assays, modified Boyden chamber trans-well assays, and cell growth assays to quantify cell migration, invasion, and proliferation in the absence or presence of Curcumin at various concentrations. Levels of the transcription factor phospho-STAT3, a potential target of Curcumin, were determined by sandwich-ELISA. Subsequent effects on transcription of genes regulating the cell cycle were analyzed by quantitative real-time PCR. Effects on apoptosis were determined by caspase assays. Curcumin potently inhibited GBM cell proliferation as well as migration and invasion in all cell lines contingent on dose. Simultaneously, levels of the biologically active phospho-STAT3 were decreased and correlated with reduced transcription of the cell cycle regulating gene c-Myc and proliferation marking Ki-67, pointing to a potential mechanism by which Curcumin slows tumor growth. Curcumin is part of the diet of millions of people every day and is without known toxic side effects. Our data show that Curcumin bears anti-proliferative, anti-migratory, and anti-invasive properties against GBM cells in vitro. These results warrant further in vivo analyses and indicate a potential role of Curcumin in the treatment of malignant gliomas.
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DOI：
10.1016/j.biocel.2008.06.010
发表时间：
2009-01
影响因子：
4
作者：
Aggarwal, Bharat B.;Harikumar, Kuzhuvelil B.
通讯作者：
Harikumar, Kuzhuvelil B.
影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
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影响因子：
4.4
作者：
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通讯作者：
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