Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements.
Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements.
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DOI：
10.1007/s00424-017-2067-y
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Lindau M
中科院分区：
文献类型：
--
作者：
Huang M;Delacruz JB;Ruelas JC;Rathore SS;Lindau M
安培法是一种记录嗜铬细胞量子释放事件的有效方法，广泛用于评估特定药物如何改变量子大小、释放动力学和早期融合孔特性。基于表面改性CMOS的电化学传感器阵列允许同时记录多个细胞。这里提出了一种可靠的、低成本的技术，用于将单细胞特异性地有效靶向到电极位点。SU-8微孔结构在芯片表面上形成图案，为电路提供绝缘，并在电极位点处捕获细胞。还结合了移位电极设计以增加微孔的尺寸和形状的灵活性。通过多巴胺注射实验验证了电极的灵敏度。尺寸略大于待捕获细胞的微孔确保了优异的单细胞靶向效率，提高了芯片上单细胞安培法测量的可靠性和效率。用微孔中捕获的活嗜铬细胞的平行记录来验证表面改性的装置。观察到没有扩散增宽的快速电流尖峰，表明捕获和记录的细胞与电极非常紧密接触。活细胞记录在单个实验中证实，尖峰参数在细胞与细胞之间显著变化，但同时记录的大量细胞提供了统计学显著性。
Amperometry is a powerful method to record quantal release events from chromaffin cells and is widely used to assess how specific drugs modify quantal size, kinetics of release and early fusion pore properties. Surface modified CMOS based electrochemical sensor arrays allow simultaneous recordings from multiple cells. A reliable, low cost technique is presented here for efficient targeting of single cells specifically to the electrode sites. An SU-8 microwell structure is patterned on the chip surface to provide insulation for the circuitry as well as cell trapping at the electrode sites. A shifted electrode design is also incorporated to increase the flexibility of the dimension and shape of the microwells. The sensitivity of the electrodes is validated by a dopamine injection experiment. Microwells with dimensions slightly larger than the cells to be trapped ensure excellent single cell targeting efficiency, increasing the reliability and efficiency for on-chip single cell amperometry measurements. The surface modified device was validated with parallel recordings of live chromaffin cells trapped in the microwells. Rapid amperometric spikes with no diffusional broadening were observed, indicating that the trapped and recorded cells were in very close contact with the electrodes. The live cell recording confirms in a single experiment that spike parameters vary significantly from cell to cell but the large number of cells recorded simultaneously, provides the statistical significance.
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影响因子：
21.3
作者：
Gong, Liang-Wei;Alvarez de Toledo, Guillermo;Lindau, Manfred
通讯作者：
Lindau, Manfred
DOI：
10.1124/jpet.111.182816
发表时间：
2011-10-01
影响因子：
3.5
作者：
Cuevas, Javier;Rodriguez, Alex;Katnik, Chris
通讯作者：
Katnik, Chris
影响因子：
5.4
作者：
Ballini M;Müller J;Livi P;Chen Y;Frey U;Stettler A;Shadmani A;Viswam V;Jones IL;Jäckel D;Radivojevic M;Lewandowska MK;Gong W;Fiscella M;Bakkum DJ;Heer F;Hierlemann A
通讯作者：
Hierlemann A
影响因子：
64.8
作者：
CHOW, RH;VONRUDEN, L;NEHER, E
通讯作者：
NEHER, E
影响因子：
7.4
作者：
Berberian, Khajak;Kisler, Kassandra;Lindau, Manfred
通讯作者：
Lindau, Manfred