Trapping and viability of swimming bacteria in an optoelectric trap.
Trapping and viability of swimming bacteria in an optoelectric trap.
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DOI：
10.1039/c5lc01559f
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发表时间：
2016-03-21
期刊：
影响因子：
6.1
通讯作者：
Wereley ST
中科院分区：
文献类型：
--
作者：
Mishra A;Maltais TR;Walter TM;Wei A;Williams SJ;Wereley ST
能够捕获和运输游动细菌的非接触操作方法可以显着帮助趋化性研究。然而，高游动速度使得捕获这些生物本身就是一项具有挑战性的任务。我们证明了一种光电技术，快速电动图像化（REP），可以有效地捕获和操纵速度大于20 μm/s的产气肠杆菌细菌。REP使用电取向、激光诱导的交流电热流和粒子电极相互作用来捕获这些细胞。与捕获不游动的细菌和惰性微球相比，我们观察到电取向对于捕获游动细胞至关重要，因为未对齐的细菌可以比径向向内的电热流更快地游动并逃离陷阱。通过评估细胞膜的完整性，我们研究了REP捕获条件，包括光辐射、激光诱导加热和电场对细胞活力的影响。当单独施加时，光辐射和激光加热对细胞的影响可以忽略不计。在标准REP捕获条件下，4分钟后只有不到2%的细胞膜受损。据我们所知，这是第一个详细描述REP捕获对细胞活力影响的研究。本研究结果为选择合适的REP参数捕获活菌提供了明确的指导。我们展示了一种动态捕获和操纵游动细菌的光电技术（REP），并表征了捕获条件对被捕获细胞活力的影响。
Non-contact manipulation methods capable of trapping and transporting swimming bacteria can significantly aid in chemotaxis studies. However, high swimming speed makes the trapping of these organisms an inherently challenging task. We demonstrate that an optoelectric technique, rapid electrokinetic patterning (REP), can effectively trap and manipulate Enterobacter aerogenes bacteria swimming at velocities greater than 20 μm/s. REP uses electro-orientation, laser-induced AC electrothermal flow, and particle-electrode interactions for capturing these cells. In contrast to trapping non-swimming bacteria and inert microspheres, we observe that electro-orientation is critical to the trapping of the swimming cells, since unaligned bacteria can swim faster than the radially inward electrothermal flow and escape the trap. By assessing the cell membrane integrity, we study the effect of REP trapping conditions, including optical radiation, laser-induced heating, and the electric field on cell viability. When applied individually, the optical radiation and laser-induced heating have negligible effect on cells. At the standard REP trapping conditions fewer than 2% of cells have a compromised membrane after four minutes. To our knowledge this is the first study detailing the effect of REP trapping on cell viability. The presented results provide a clear guideline on selecting suitable REP parameters for trapping living bacteria. We demonstrate an optoelectric technique (REP) for dynamic trapping and manipulation of swimming bacteria, and characterize the effect of trapping conditions on the viability of the trapped cells.
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影响因子：
3.9
作者：
Kumar, Aloke;Kwon, Jae-Sung;Wereley, Steven T.
通讯作者：
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