A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emission.
A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emission.
复制标题
DOI：
10.1002/anie.201105653
复制
发表时间：
2012-04-02
影响因子：
16.6
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Conley, Nicholas R.;Dragulescu-Andrasi, Anca;Rao, Jianghong;Moerner, W. E.
生物发光成像（BLI）利用产生光的荧光素酶，最常见的是萤火虫荧光素酶（FLuc）， Renilla reniformis荧光素酶或Gaussia荧光素酶与相应的底物结合活体对象的无创BLI已成为癌症生物学研究的常规技术，因为它可以监测基因表达、基因传递、肿瘤生长、酶活性、对实验药物治疗的反应以及蛋白质-蛋白质相互作用。体内BLI实验的主要限制是光被组织吸收和散射，这导致在600 nm以下发射的BL信号有很强的衰减天然荧光素底物（FLuc, λem, max= 553 - 559nm）发射的黄绿色BL随组织深度的增加而显著降低。因此，该系统的应用主要局限于浅层深处的小动物。为了克服这一限制，利用随机诱变技术选择了天然FLuc的红移变体大量的努力也被用于开发d -荧光素[6]的类似物，产生更长的波长光，这是一种正交的酶工程方法。最近的一份报告描述了d -荧光素类似物的发展，它在近红外区域发射。[6e]该类似物是氨基荧光素- cy5缀合物，基于生物发光共振能量转移（BRET）。然而，这种类型的修饰改变了底物的细胞摄取特性，并可能改变其在体内的生物分布。这里我们描述一个
Bioluminescence imaging (BLI) makes use of light-generating luciferase enzymes, most commonly firefly luciferase (FLuc), Renilla reniformis luciferase or Gaussia luciferase in combination with their appropriate substrates.[1] Noninvasive BLI of living subjects has become a routine technique in cancer biology research because it enables monitoring of gene expression, gene delivery, tumor growth, enzyme activity, response to experimental drug therapies, and protein-protein interactions.[2]The major limitation in in vivo BLI experiments is absorption and scattering of light by tissue, which results in strong attenuation of BL signal emitted below 600 nm.[3] The yellow-green BL emission from the native FLuc (D-luciferin substrate, λem, max= 553–559 nm),[4] which is the most red-shifted native bioluminescent system, decreases substantially with tissue depth. Consequently applications of this system are restricted mainly to small animals at superficial depths. To overcome this limitation, red-shifted variants of native FLuc have been selected using random mutagenesis.[5] Considerable efforts have also been directed toward the development of analogues of D-luciferin [6] that produce longer wavelength light, an orthogonal approach to enzyme engineering. A recent report describes the development of a D-luciferin analogue, which emits in the near-infrared region.[6e] This analogue is an aminoluciferin-Cy5 conjugate and is based on bioluminescence resonance energy transfer (BRET). This type of modification, however, alters the cellular uptake properties of the substrate and likely changes its biodistribution in vivo. Here we describe a
登录
查看更多内容
影响因子：
4.1
作者：
Lembert, N
通讯作者：
Lembert, N
影响因子：
2.9
作者：
Branchini, BR;Southworth, TL;Roda, A
通讯作者：
Roda, A
DOI：
10.1002/cber.19620950742
发表时间：
1962-01-01
期刊：
CHEMISCHE BERICHTE-RECUEIL
影响因子：
--
作者：
BAGANZ, H;DOMASCHKE, L
通讯作者：
DOMASCHKE, L
影响因子：
4.3
作者：
DEMARCO, C;RINALDI, A;DERNINI, S
通讯作者：
DERNINI, S
影响因子：
1.2
作者：
Mieville, Pascal;Jannin, Sami;Bodenhausen, Geoffrey
通讯作者：
Bodenhausen, Geoffrey