Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates.
Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates.
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DOI：
10.1093/ve/veab062
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发表时间：
2021
期刊：
影响因子：
5.3
通讯作者：
Bataille A
中科院分区：
文献类型：
--
作者：
Benfield CTO;Hill S;Shatar M;Shiilegdamba E;Damdinjav B;Fine A;Willett B;Kock R;Bataille A
小反刍兽疫病毒（PPRV）在家养和野生有蹄类动物中引起疾病，是全球根除计划的目标，并威胁生物多样性。了解PPRV在野生动物中的流行病学和进化是重要的，但由于缺乏野生动物来源的PPRV基因组而受到阻碍。在这项研究中，完整的PPRV基因组来自2016-2017年PPRV爆发的三只蒙古赛加羚羊，一只西伯利亚山羊和一只甲状腺瞪羚。系统发育分析表明，自2013年以来，蒙古和中国PPRV的野生动物和牲畜源基因组密切相关并散布。2013年以来，蒙古野生动物和家畜中的PPRV属于单系群，属于中国家畜和野生动物中的IV系PPRV分支。离散扩散分析发现强有力的支持，PPRV从中国传播到蒙古，和地理分析表明新疆省是最可能的起源，虽然PPRV的基因组监测是穷人和缺乏从其他地区的采样可能会使这一结果产生偏差。所有蒙古PPRV基因组的最近共同祖先（TMRCA）时间为2015年6月（95%最高后验密度（HPD）：2014年8月至2016年3月），蒙古野生动物来源的PPRV为2016年5月（95% HPD：2015年10月至2016年10月）。这表明，在2016年8月首次报告爆发之前，PPRV在蒙古至少6个月内未被发现，野生动物可能在2016年10月开始牲畜接种疫苗之前受到感染。最后，遗传变异和积极选择的网站被确定，可能与PPRV出现在蒙古野生动物。这项研究是第一个对来自野生动物爆发的多个宿主物种的多个PPRV基因组进行测序。需要额外的完整PPRV基因组和来自牲畜-野生动物界面的相关元数据来增强分子流行病学的力量，支持PPRV根除，并保护整个有蹄类动物群落的健康。
Peste des petits ruminants virus (PPRV) causes disease in domestic and wild ungulates, is the target of a Global Eradication Programme, and threatens biodiversity. Understanding the epidemiology and evolution of PPRV in wildlife is important but hampered by the paucity of wildlife-origin PPRV genomes. In this study, full PPRV genomes were generated from three Mongolian saiga antelope, one Siberian ibex, and one goitered gazelle from the 2016–2017 PPRV outbreak. Phylogenetic analysis showed that for Mongolian and Chinese PPRV since 2013, the wildlife and livestock-origin genomes were closely related and interspersed. There was strong phylogenetic support for a monophyletic group of PPRV from Mongolian wildlife and livestock, belonging to a clade of lineage IV PPRV from livestock and wildlife from China since 2013. Discrete diffusion analysis found strong support for PPRV spread into Mongolia from China, and phylogeographic analysis indicated Xinjiang Province as the most likely origin, although genomic surveillance for PPRV is poor and lack of sampling from other regions could bias this result. Times of most recent common ancestor (TMRCA) were June 2015 (95 per cent highest posterior density (HPD): August 2014 to March 2016) for all Mongolian PPRV genomes and May 2016 (95 per cent HPD: October 2015 to October 2016) for Mongolian wildlife-origin PPRV. This suggests that PPRV was circulating undetected in Mongolia for at least 6 months before the first reported outbreak in August 2016 and that wildlife were likely infected before livestock vaccination began in October 2016. Finally, genetic variation and positively selected sites were identified that might be related to PPRV emergence in Mongolian wildlife. This study is the first to sequence multiple PPRV genomes from a wildlife outbreak, across several host species. Additional full PPRV genomes and associated metadata from the livestock–wildlife interface are needed to enhance the power of molecular epidemiology, support PPRV eradication, and safeguard the health of the whole ungulate community.
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影响因子：
3.7
作者：
Bieringer M;Han JW;Kendl S;Khosravi M;Plattet P;Schneider-Schaulies J
通讯作者：
Schneider-Schaulies J
影响因子：
2.8
作者：
Aziz-ul-Rahman;Munir, Muhammad;Shabbir, Muhammad Zubair
通讯作者：
Shabbir, Muhammad Zubair
影响因子：
1.7
作者：
Aziz-ul-Rahman;Wensman, Jonas Johansson;Rossiter, Paul
通讯作者：
Rossiter, Paul
影响因子：
2.4
作者：
Bao, Jingyue;Wang, Zhiliang;Qi, Lin
通讯作者：
Qi, Lin
影响因子：
3.1
作者：
Couacy-Hymann, E;Roger, F;Diallo, A
通讯作者：
Diallo, A