ArticleEaf 3 Chromodomain Interaction with Methylated H 3K 36 Links Histone Deacetylation to Pol II Elongation
ArticleEaf 3 Chromodomain Interaction with Methylated H 3K 36 Links Histone Deacetylation to Pol II Elongation
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ArticleEaf 3 染色质结构域与甲基化 H 3K 36 的相互作用将组蛋白脱乙酰化与 Pol II 延伸联系起来
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通讯作者：
K. Struhl
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作者：
A. Joshi;K. Struhl
Eaf3是NuA4组蛋白乙酰化酶和Rpd3组蛋白去乙酰化酶复合体中的一个组分，对酿酒酵母组蛋白乙酰化的整体模式具有重要作用。编码区的优先脱乙酰基需要Set2的Eaf3染色域和H3-K36甲基化。Eaf3染色域与甲基化的H3-K36多肽相互作用，表明这种相互作用导致了Rpd3复合体对编码区30个部分的优先结合和组蛋白去乙酰化。然而，Eaf3染色域和H3-K36甲基化并不显著影响启动子的乙酰化，这表明Eaf3具有不同的功能，可能是在NuA4复合体中。最后，Eaf3以类似于FACT和Spt6的方式抑制了mRNA编码区的内部启动。我们的结果将编码区的优先去乙酰化模式与RNA聚合酶II C末端结构域的H3-K36甲基化和磷酸化的潜在模式联系起来，最终与转录延长后抑制的染色质结构恢复的机制有关。
Eaf3, a component of the NuA4 histone acetylase and Rpd3 histone deacetylase complexes, is important for the global pattern of histone acetylation in Saccharomyces cerevisiae. Preferential deacetylation of coding regions requires the Eaf3 chromodomain and H3-K36 methylation by Set2. The Eaf3 chromodomain interacts with methylated H3-K36 peptides, suggesting that this interaction leads to preferential association and histone deacetylation of the 30 portions of coding regions by the Rpd3 complex. However, the Eaf3 chromodomain and H3-K36 methylation do not significantly affect acetylation at promoters, suggesting that Eaf3 has a distinct function, presumably in the NuA4 complex. Lastly, Eaf3 inhibits internal initiation within mRNA coding regions in a manner similar to FACT and Spt6. Our results link the pattern of preferential deacetylation at coding regions to the underlying patterns of H3-K36 methylation and phosphorylation of the RNA polymerase II C-terminal domain, and ultimately to the mechanism by which repressive chromatin structure is restored after transcriptional elongation.
影响因子：
16
作者：
Suka, N;Suka, Y;Grunstein, M
通讯作者：
Grunstein, M
影响因子：
16
作者：
Ng, HH;Robert, F;Struhl, K
通讯作者：
Struhl, K
影响因子：
3.5
作者：
Bertram, MJ;Pereira-Smith, OM
通讯作者：
Pereira-Smith, OM