Dynamic analysis of vascular morphogenesis using transgenic quail embryos.
Dynamic analysis of vascular morphogenesis using transgenic quail embryos.
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DOI：
10.1371/journal.pone.0012674
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发表时间：
2010-09-14
期刊：
影响因子：
3.7
通讯作者：
Lansford R
中科院分区：
文献类型：
--
作者：
Sato Y;Poynter G;Huss D;Filla MB;Czirok A;Rongish BJ;Little CD;Fraser SE;Lansford R
生物学家面临的一个最不为人所知但又最核心的问题是，最初简单的细胞簇或片状细胞群是如何组装成高度复杂的三维功能组织和器官的。由于氧气扩散的限制，血管是所有羊膜组织和器官中必不可少且无处不在的存在。血管发生是内皮细胞（EC）前体重新自组装成内皮管，是血管形成的第一步。静态成像和体外模型完全不足以捕捉体内血管模式形成的许多方面，因为血管发生涉及内皮细胞和血管形成的动态变化，在胚胎中改变大小和形状。我们已经产生了Tie1转基因鹌鹑系Tg(Tie1:H2B-eYFP)，在其所有内皮细胞中表达H2B-eYFP，这使得研究早期胚胎血管形态发生具有前所未有的清晰度和洞察力。通过结合分子遗传学的力量和动态成像的优雅，我们遵循内皮细胞在空间和时间上的精确模式。我们发现，在血管丛内的血管形成过程中，内皮细胞独立运动形成血管的雏形，同时与原肠泌乳组织一起向胚胎中线流动。主动脉是形成其背侧区域的体细胞来源的内皮细胞和形成其腹侧区域的内脏来源的内皮细胞的复合物。形成主动脉背侧区域的内皮细胞在向侧运动时表现出可变的中外侧运动；那些在更腹侧的区域显示出显著的从外侧到内侧的运动。目前的结果为研究血管发育的机械、分子和细胞机制的相对作用提供了一个强有力的方法。在过去的研究中，小鼠分子遗传工具的优势被成像和摄动研究所需的有限实验可及性所抵消。禽类胚胎提供了所需的可获得性，但遗传资源很少。建立带有标记内皮的转基因鹌鹑是基于禽类胚胎在先前血管发育研究中所起的重要作用。
One of the least understood and most central questions confronting biologists is how initially simple clusters or sheet-like cell collectives can assemble into highly complex three-dimensional functional tissues and organs. Due to the limits of oxygen diffusion, blood vessels are an essential and ubiquitous presence in all amniote tissues and organs. Vasculogenesis, the de novo self-assembly of endothelial cell (EC) precursors into endothelial tubes, is the first step in blood vessel formation. Static imaging and in vitro models are wholly inadequate to capture many aspects of vascular pattern formation in vivo, because vasculogenesis involves dynamic changes of the endothelial cells and of the forming blood vessels, in an embryo that is changing size and shape. We have generated Tie1 transgenic quail lines Tg(tie1:H2B-eYFP) that express H2B-eYFP in all of their endothelial cells which permit investigations into early embryonic vascular morphogenesis with unprecedented clarity and insight. By combining the power of molecular genetics with the elegance of dynamic imaging, we follow the precise patterning of endothelial cells in space and time. We show that during vasculogenesis within the vascular plexus, ECs move independently to form the rudiments of blood vessels, all while collectively moving with gastrulating tissues that flow toward the embryo midline. The aortae are a composite of somatic derived ECs forming its dorsal regions and the splanchnic derived ECs forming its ventral region. The ECs in the dorsal regions of the forming aortae exhibit variable mediolateral motions as they move rostrally; those in more ventral regions show significant lateral-to-medial movement as they course rostrally. The present results offer a powerful approach to the major challenge of studying the relative role(s) of the mechanical, molecular, and cellular mechanisms of vascular development. In past studies, the advantages of the molecular genetic tools available in mouse were counterbalanced by the limited experimental accessibility needed for imaging and perturbation studies. Avian embryos provide the needed accessibility, but few genetic resources. The creation of transgenic quail with labeled endothelia builds upon the important roles that avian embryos have played in previous studies of vascular development.
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DOI：
10.1073/pnas.92.17.7657
发表时间：
1995-08-15
影响因子：
11.1
作者：
DRAKE, CJ;LITTLE, CD
通讯作者：
LITTLE, CD
影响因子：
4.8
作者：
Iljin, K;Petrova, TV;Alitalo, K
通讯作者：
Alitalo, K
DOI：
10.1073/pnas.93.21.11382
发表时间：
1996-10-15
影响因子：
11.1
作者：
Naldini, L;Blomer, U;Verma, IM
通讯作者：
Verma, IM
影响因子：
6.9
作者：
Huss, David;Poynter, Greg;Lansford, Rusty
通讯作者：
Lansford, Rusty
影响因子：
56.9
作者：
Lois, C;Hong, EJ;Baltimore, D
通讯作者：
Baltimore, D