Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
复制标题
DOI：
10.1371/journal.pone.0041760
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Charalampaki P
中科院分区：
文献类型：
--
作者：
Foersch S;Heimann A;Ayyad A;Spoden GA;Florin L;Mpoukouvalas K;Kiesslich R;Kempski O;Goetz M;Charalampaki P
手术中早期发现和评估脑肿瘤是准确切除的关键。目前在手术中经常进行冷冻。共聚焦激光显微内镜（CLE）是一种新颖的技术，允许在体内组织成像的微型内镜探针，具有优异的分辨率。本研究的目的是评估CLE在不同类型和模型的颅内肿瘤的体内诊断价值。研究了两种不同的同种异体C6胶质瘤细胞系在大鼠体内的组织形态学变化。一个细胞系表达EYFP，另一个细胞系用荧光染料（荧光素、吖啶黄、fitc -葡聚糖和吲哚菁绿）染色。为了评价其在患者中的应用前景，我们对15例颅内肿瘤（多形性胶质母细胞瘤、脑膜瘤、颅咽管瘤、听神经瘤、脑转移瘤、成神经管细胞瘤、表皮样瘤）的新鲜手术切除标本进行了检查。与样本相邻的健康脑组织作为对照。CLE产生了高质量的正常脑组织和肿瘤的组织形态学。不同的荧光剂显示组织和细胞结构的不同方面（核模式，轴突通路，出血）。基于组织和细胞结构，CLE区分肿瘤与健康脑组织很容易，与组织病理学有很好的相似性。共聚焦激光内窥镜可以在高分辨率下立即对正常脑组织和肿瘤脑组织进行体内成像。该技术可在神经外科和神经病理学中转化为科学和临床应用。这将有助于肿瘤边缘的筛查和恶性脑肿瘤的手术切除，并为肿瘤和其他神经系统疾病的体内分子成像打开大门。
Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Aim of the current study was to evaluate CLE for in vivo diagnosis in different types and models of intracranial neoplasia. In vivo histomorphology of healthy brains and two different C6 glioma cell line allografts was evaluated in rats. One cell line expressed EYFP, the other cell line was used for staining with fluorescent dyes (fluorescein, acriflavine, FITC-dextran and Indocyanine green). To evaluate future application in patients, fresh surgical resection specimen of human intracranial tumors (n = 15) were examined (glioblastoma multiforme, meningioma, craniopharyngioma, acoustic neurinoma, brain metastasis, medulloblastoma, epidermoid tumor). Healthy brain tissue adjacent to the samples served as control. CLE yielded high-quality histomorphology of normal brain tissue and tumors. Different fluorescent agents revealed distinct aspects of tissue and cell structure (nuclear pattern, axonal pathways, hemorrhages). CLE discrimination of neoplastic from healthy brain tissue was easy to perform based on tissue and cellular architecture and resemblance with histopathology was excellent. Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. The technology might be transferred to scientific and clinical application in neurosurgery and neuropathology. It may become helpful to screen for tumor free margins and to improve the surgical resection of malignant brain tumors, and opens the door to in vivo molecular imaging of tumors and other neurologic disorders.
登录
查看更多内容
影响因子：
4.8
作者：
Sankar, Tejas;Delaney, Peter M.;Preul, Mark C.
通讯作者：
Preul, Mark C.
影响因子：
7.7
作者：
Knauer, Shirley K.;Bier, Carolin;Stauber, Roland H.
通讯作者：
Stauber, Roland H.
影响因子：
4.1
作者：
Martirosyan, Nikolay L.;Cavalcanti, Daniel D.;Preul, Mark C.
通讯作者：
Preul, Mark C.
影响因子：
4.1
作者：
Lacroix, M;Abi-Said, D;Sawaya, R
通讯作者：
Sawaya, R
影响因子：
1.5
作者：
Hale, Sarah L.;Andrews-Jones, Lydia;Little, Peter B.
通讯作者：
Little, Peter B.