Comparative Transcriptome Analyses Reveal a Special Glucosinolate Metabolism Mechanism in Brassica alboglabra Sprouts.
Comparative Transcriptome Analyses Reveal a Special Glucosinolate Metabolism Mechanism in Brassica alboglabra Sprouts.
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比较转录组分析揭示了甘蓝芽中特殊的芥子油苷代谢机制
DOI：
10.3389/fpls.2016.01497
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发表时间：
2016
影响因子：
5.6
通讯作者：
Lai Z
中科院分区：
文献类型：
--
作者：
Guo R;Huang Z;Deng Y;Chen X;XuHan X;Lai Z
甘蓝含有丰富的植物化学物质，尤其是硫代葡萄糖甙（GS）。已使用各种方法来提高芽中GS含量。然而，芽中GS代谢的分子基础仍然是一个悬而未决的问题。在此，我们采用RNA-seq分析来比较高GS（JL-08）和低GS（JL-09）芥蓝芽的转录组。产生配对末端Illumina RNA-seq读数并映射到甘蓝参考基因组。分析JL-08和JL-09之间差异表达的基因。其中，与JL-08相比，JL-09中有1477个基因表达上调，1239个基因表达下调。对这些差异表达基因的富集分析表明，在京都基因组数据库中，GS生物合成途径的富集因子最小，Q值最高，表明JL-08和JL-09的主要代谢差异是GS生物合成途径。37个基因的测序数据被注释为参与GS的生物合成，降解和调控，其中11个差异表达在JL-08和JL-09。高GS JL-08中GS降解酶黑芥子酶的表达水平低于低GS JL-09。令人惊讶的是，在高GS JL-08中，GS生物合成基因的表达水平也低于低GS JL-09。由于芽中GS含量是由种子贮藏GS动员、从头合成、降解和额外运输的动态平衡决定的，因此，提高芽中GS含量的努力应着眼于提高种子中GS含量或降低黑芥子酶活性，而不是提高芽中GS生物合成基因的表达水平。
Brassica sprouts contain abundant phytochemicals, especially glucosinolates (GSs). Various methods have been used to enhance GS content in sprouts. However, the molecular basis of GS metabolism in sprouts remains an open question. Here we employed RNA-seq analysis to compare the transcriptomes of high-GS (JL-08) and low-GS (JL-09) Brassica alboglabra sprouts. Paired-end Illumina RNA-seq reads were generated and mapped to the Brassica oleracea reference genome. The differentially expressed genes were analyzed between JL-08 and JL-09. Among these, 1477 genes were up-regulated and 1239 down-regulated in JL-09 compared with JL-08. Enrichment analysis of these differentially expressed genes showed that the GS biosynthesis had the smallest enrichment factor and the highest Q-value of all metabolic pathways in Kyoto Encyclopedia of Genes and Genomes database, indicating the main metabolic difference between JL-08 and JL-09 is the GS biosynthetic pathway. Thirty-seven genes of the sequenced data were annotated as putatively involved in GS biosynthesis, degradation, and regulation, of which 11 were differentially expressed in JL-08 and JL-09. The expression level of GS degradation enzyme myrosinase in high-GS JL-08 was lower compared with low-GS JL-09. Surprisingly, in high-GS JL-08, the expression levels of GS biosynthesis genes were also lower than those in low-GS JL-09. As the GS contents in sprouts are determined by dynamic equilibrium of seed stored GS mobilization, de novo synthesis, degradation, and extra transport, the result of this study leads us to suggest that efforts to increase GS content should focus on either raising GS content in seeds or decreasing myrosinase activity, rather than improving the expression level of GS biosynthesis genes in sprouts.
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西兰花（Brassica oleracea var. italic）种子和芽中转录组和芥子油苷代谢的 RNA 测序分析
影响因子：
3.7
作者：
Gao J;Yu X;Ma F;Li J
通讯作者：
Li J
影响因子：
9.4
作者：
Gigolashvili, Tamara;Engqvist, Martin;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
7.4
作者：
Celenza, JL;Quiel, JA;Bender, J
通讯作者：
Bender, J
影响因子：
7.2
作者：
Chen, SX;Glawischnig, E;Halkier, BA
通讯作者：
Halkier, BA
影响因子：
7.2
作者：
Gigolashvili, Tamara;Berger, Bettina;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo