Mice lacking galectin-3 (Lgals3) function have decreased home cage movement.
Mice lacking galectin-3 (Lgals3) function have decreased home cage movement.
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DOI：
10.1186/s12868-018-0428-x
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发表时间：
2018-05-02
期刊：
影响因子：
2.4
通讯作者：
Bonasera SJ
中科院分区：
文献类型：
--
作者：
Chaudoin TR;Bonasera SJ
半乳糖凝集素是一个大家族的蛋白质进化识别特定的碳水化合物部分。鉴于模式识别过程对多种生物学任务的重要性，包括CNS发育和免疫识别，我们研究了缺乏半乳糖凝集素-3（Lgals 3）功能的小鼠的家庭笼行为表型。使用能够以毫秒时间和0.5 cm空间分辨率检查进食、饮水和运动的复杂监测装置，我们连续17天观察了10只野生型雄性C57 BL/6 J和10只Lgals 3组成型敲除（Lgals 3 −/−;两个队列均为2-3个月大）小鼠的日常行为模式。我们在6-7个月大时对该队列进行了第二次行为评估。在两个年龄段，Lgals 3 −/−小鼠与野生型对照组相比表现出较少的运动。Lgals 3 −/−小鼠的向前运动和原地运动行为都减少了，这是由于回合数、起始速率和持续时间减少。我们还注意到Lgals 3 −/−小鼠的行为昼夜节律受到干扰，与野生型相比，两个年龄段的小鼠在进食、饮水和运动的日常表现方面表现出更大的变异性（通过Lomb-Scargle分析评估）。Lgals 3执行的碳水化合物识别任务可能是参与运动行为生成和控制的CNS结构适当发育所必需的。本文的在线版本（10.1186/s12868-018-0428-x）包含补充材料，可供授权用户使用。
Galectins are a large family of proteins evolved to recognize specific carbohydrate moieties. Given the importance of pattern recognition processes for multiple biological tasks, including CNS development and immune recognition, we examined the home cage behavioral phenotype of mice lacking galectin-3 (Lgals3) function. Using a sophisticated monitoring apparatus capable of examining feeding, drinking, and movement at millisecond temporal and 0.5 cm spatial resolutions, we observed daily behavioral patterns from 10 wildtype male C57BL/6J and 10 Lgals3 constitutive knockout (Lgals3−/−; both cohorts aged 2–3 months) mice over 17 consecutive days. We performed a second behavioral assessment of this cohort at age 6–7 months. At both ages, Lgals3−/− mice demonstrated less movement compared to wildtype controls. Both forward locomotion and movement-in-place behaviors were decreased in Lgals3−/− mice, due to decreased bout numbers, initiation rates, and durations. We additionally noted perturbation of behavioral circadian rhythms in Lgals3−/− mice, with mice at both ages demonstrating greater variability in day-to-day performance of feeding, drinking, and movement (as assessed by Lomb-Scargle analysis) compared to wildtype. Carbohydrate recognition tasks performed by Lgals3 may be required for appropriate development of CNS structures involved in the generation and control of locomotor behavior. The online version of this article (10.1186/s12868-018-0428-x) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Haudek, Kevin C.;Spronk, Kimberly J.;Voss, Patricia G.;Patterson, Ronald J.;Wang, John L.;Arnoys, Eric J.
通讯作者：
Arnoys, Eric J.
DOI：
10.1073/pnas.87.18.7324
发表时间：
1990-09-01
影响因子：
11.1
作者：
CHERAYIL, BJ;CHAITOVITZ, S;PILLAI, S
通讯作者：
PILLAI, S
影响因子：
25
作者：
Glynn, Marian W.;Elmer, Bradford M.;Garay, Paula A.;Liu, Xiao-Bo;Needleman, Leigh A.;El-Sabeawy, Faten;McAllister, A. Kimberley
通讯作者：
McAllister, A. Kimberley
影响因子：
4.4
作者：
Jeon, Sae-Bom;Yoon, Hee Jung;Park, Eun Jung
通讯作者：
Park, Eun Jung
影响因子：
10.7
作者：
Houzelstein, D;Gonçalves, IR;Poirier, F
通讯作者：
Poirier, F