Newly established cell lines fromDrosophila larval CNS express neural specific characteristics
Newly established cell lines fromDrosophila larval CNS express neural specific characteristics
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新建立的果蝇幼虫中枢神经系统细胞系表达神经特异性特征
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Tadashi Miyake
中科院分区：
文献类型：
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作者：
K. Ui;S. Nishihara;Masafumi Sakuma;Shin Togashi;Ryu Ueda;Yuhei Miyata;Tadashi Miyake
从果蝇（Drosophilamelanogaster）3龄幼虫的中枢神经系统中建立了8个连续细胞系（命名为ML-DmBG 1 ~ 8）。使用ML-DmBG 2进行单菌落分离，获得6个菌落克隆。所有这些都对辣根过氧化物酶的抗体产生了反应，辣根过氧化物酶是昆虫的一种神经元标记。乙酰胆碱是果蝇中一种已知的神经递质，通过高效液相色谱法在三个克隆中检测到。因此，可以得出结论，所建立的克隆是起源于幼虫中枢神经系统的神经细胞。其中，观察到一些变化的形态，乙酰胆碱含量，和抗HRP的反应。这种变化可能反映了组成中枢神经系统的细胞的异质性。
SummaryFrom the central nervous system ofDrosophila melanogaster 3rd instar larvae, eight continuous cell lines have been established (named ML-DmBG1 to 8). Using ML-DmBG2, single colony isolation was carried out and six colonial clones were obtained. All reacted to the antibody to horseradish peroxidase, which is a neuronal marker in insects. Acetylcholine, a known neurotransmitter inDrosophila, was detected in three of the colonial clones by high performance liquid chromatography. Therefore, it is concluded that the established colonial clones are neural cells originating in the larval central nervous system. Among them, some variation was observed with respect to morphology, acetylcholine content, and reactivity to anti-HRP. The variation may reflect the heterogeneity of cells composing the central nervous system.
巨型果蝇神经元从胞质分裂停滞的胚胎神经母细胞分化而来。
DOI：
10.1002/neu.480210310
发表时间：
1990
期刊：
Journal of neurobiology
影响因子：
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作者：
Wu,CF;Sakai,K;Saito,M;Hotta,Y
通讯作者：
Hotta,Y