Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.
Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.
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Brg1 的过度表达通过抗氧化应激作用减轻肝缺血/再灌注引起的急性肺损伤
DOI：
10.1155/2017/8787392
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发表时间：
2017
影响因子：
--
通讯作者：
Hei Z
中科院分区：
文献类型：
--
作者：
Ge M;Chen C;Yao W;Zhou S;Huang F;Cai J;Hei Z
目的探讨Brahma相关基因1（Brg 1）过表达对肝缺血再灌注（HIR）所致肺损伤的保护作用及其机制。方法采用巨细胞病毒转基因Brg 1过表达（CMV-Brg 1）小鼠和野生型（WT）C57 BL/6小鼠进行HIR。评估肺组织学、氧化损伤标记物和肺中的抗氧化酶浓度。Western blotting法检测Brg 1、核因子红细胞2相关因子2（Nrf 2）、血红素加氧酶-1（HO-1）和NAD（P）H：醌氧化还原酶1（NQO 1）在肺组织中的蛋白表达水平。结果WT组肺组织病理学检查显示，肺损伤在HIR后6 h达到高峰。同时，肺活性氧（ROS）和8-异前列腺素（8-isoprostane）水平显著升高。Brg 1蛋白表达在6 h时降至最低。Brg 1过表达可减轻肺损伤，降低氧化产物的含量，包括8-异前列腺素和ROS的水平，以及4-羟基壬烯醛（4-HNE）和8-氧代-2 ′-脱氧鸟苷（8-OHdG）阳性细胞的百分比。Brg 1过表达增加了Nrf 2的表达和核转位，并激活了抗氧化酶。此外，它还能降低炎症因子的表达。结论Brg 1过表达可能通过Nrf 2途径减轻HIR诱导的氧化性肺损伤。
Aim To investigate whether overexpression of Brahma-related gene-1 (Brg1) can alleviate lung injury induced by hepatic ischemia/reperfusion (HIR) and its precise mechanism. Methods Cytomegalovirus-transgenic Brg1-overexpressing (CMV-Brg1) mice and wild-type (WT) C57BL/6 mice underwent HIR. Lung histology, oxidative injury markers, and antioxidant enzyme concentrations in the lung were assessed. The protein expression levels of Brg1, nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase 1 (NQO1) in the lung were analyzed by Western blotting. Results In the WT group, histopathological analysis revealed that lung damage peaked at 6 h after HIR. Meanwhile, the lung reactive oxygen species (ROS) and 8-isoprostane levels were significantly increased. The protein expression of Brg1 in lung tissue decreased to a minimum at 6 h. Overexpression of Brg1 alleviated lung injury and decreased the amounts of oxidative products, including the levels of 8-isoprostane and ROS, as well as the percentage of positive cells for 4-hydroxynonenal (4-HNE) and 8-oxo-2′-deoxyguanosine (8-OHdG). Brg1 overexpression increased the expression and nuclear translocation of Nrf2 as well as activated the antioxidases. In addition, it decreased the expression of inflammatory factors. Conclusion Overexpression of Brg1 alleviates oxidative lung injury induced by HIR, likely through the Nrf2 pathway.
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影响因子：
15.3
作者：
Cannistra, Marco;Ruggiero, Michele;Nardo, Bruno
通讯作者：
Nardo, Bruno
血红素诱导血红素加氧酶-1 保护肺免受原位自体肝移植引起的大鼠急性肺损伤
影响因子：
7.4
作者：
Chi X;Guo N;Yao W;Jin Y;Gao W;Cai J;Hei Z
通讯作者：
Hei Z
影响因子：
4.3
作者：
Jiang, An;Liu, Chang;Lv, Yi
通讯作者：
Lv, Yi
影响因子：
15.9
作者：
COLLETTI, LM;KUNKEL, SL;STRIETER, RM
通讯作者：
STRIETER, RM
影响因子：
25.7
作者：
Jaeschke H;Ramachandran A
通讯作者：
Ramachandran A